Mouse LIX/CXCL5 ELISA Kit
Validation Data Gallery
Product Information
KE10070 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse LIX/CXCL5 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse LIX/CXCL5 . The assay recognizes mouse LIX/CXCL5. An antibody specific for mouse LIX/CXCL5 has been pre-coated onto the microwells. The mouse LIX/CXCL5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse LIX/CXCL5 is added to detect the captured mouse LIX/CXCL5 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse LIX/CXCL5 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernates, Tissue lysates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 19.3 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20311 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 113% | 102%-124% |
| Tissue lysates | 103% | 85%-119% |
| Cell culture supernates | 106% | 82%-113% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 930.7 | 78.7 | 8.5 |
| 2 | 20 | 223.9 | 6.9 | 3.1 |
| 3 | 20 | 47.7 | 4.8 | 10.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 787.0 | 50.9 | 6.5 |
| 2 | 24 | 180.4 | 8.4 | 4.7 |
| 3 | 24 | 31.4 | 1.8 | 5.7 |
Background Information
LPS-induced CXC chemokine (LIX) is a murine chemokine similar to two human chemokines, ENA-78 (CXCL5) and GCP-2 (CXCL6). Mouse LIX can be produced by many immune cells, such as macrophages, eosinophils , as well as non-immune cells including mesothelial cells, and fibroblasts. Increased serum LIX has been observed in obesity and insulin resistance in mice and humans and is associated with complications of diabetes including nephropathy and atherosclerosis. It is mainly produced when induced by LPS, IL-17 and/or TNF-α. According to the previous studies, cancer cell autonomous secretion of LIX as sufficient to drive infiltration of mature, protumorigenic neutrophils in a mouse model of non-small cell lung cancer (NSCLC).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C-X-C motif chemokine 5,Cxcl5,Cytokine LIX,GCP-2(1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Cell Metab Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity | ||
Adv Sci (Weinh) Targeting Deltex E3 Ubiquitin Ligase 2 Inhibits Tumor-associated Neutrophils and Sensitizes Hepatocellular Carcinoma Cells to Immunotherapy | ||
Sleep Med Platelet-activating cytokines potentially associated with MASLD-induced liver injury significantly decreased following CPAP therapy: A translational study using a fatty liver mouse model | ||
Med Nanodrug-bacteria conjugates-mediated oncogenic collagen depletion enhances immune checkpoint blockade therapy against pancreatic cancer |
