Mouse MCP-1 ELISA Kit
Cat No : KE10006
Synonyms
AI323594, Ccl2, chemokine (C C motif) ligand 2, HC11, JE, MCAF, MCP 1, Mcp1, MCP-1, Scya2, Sigje, SMC CF
Validation Data Gallery
Product Information
KE10006 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MCP-1 ELISA kit is to be used to detect and quantify protein levels of endogenous MCP-1. The assay recognizes mouse MCP-1. An antibody specific for MCP-1 has been pre-coated onto the microwells. The MCP-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for MCP-1 is added to detect the captured MCP-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse MCP-1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1.3 pg/mL |
| Range | 3.13-200 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20296 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 91% | 76%-127% |
| Cell culture supernatants | 102% | 85%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 196.2 | 17.4 | 8.8 |
| 2 | 20 | 52.3 | 2.9 | 5.5 |
| 3 | 20 | 11.2 | 0.7 | 6.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 110.1 | 9.2 | 8.4 |
| 2 | 24 | 33.1 | 2.6 | 8.0 |
| 3 | 24 | 8.5 | 0.7 | 8.4 |
Background Information
Monocyte chemotactic protein 1 (Mcp1; also known as Ccl2), is chemotactic for monocyte/macrophage, B-cell, and T-lymphocyte, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AI323594, Ccl2, chemokine (C C motif) ligand 2, HC11, JE, MCAF, MCP 1, Mcp1, MCP-1, Scya2, Sigje, SMC CF |
Publications
| Species | Sample Type | Title |
|---|---|---|
Bone Res Osteopontin deficiency promotes cartilaginous endplate degeneration by enhancing the NF-κB signaling to recruit macrophages and activate the NLRP3 inflammasome | ||
Nat Commun Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH) | ||
ACS Appl Mater Interfaces Prussian Blue Nanozymes Prevent Anthracycline-Induced Liver Injury by Attenuating Oxidative Stress and Regulating Inflammation. | ||
Adv Healthc Mater Enhanced Antibacterial And Wound Healing Using A Metal-Organic Cluster Inspired by Artificial Photosynthesis | ||
Int J Biol Sci Olfactomedin-4 deletion exacerbates DSS-induced colitis through a matrix metalloproteinase-9-dependent mechanism | ||
Cancer Lett SIGLEC15 amplifies immunosuppressive properties of tumor-associated macrophages in pancreatic cancer. |