Mouse MIF ELISA Kit
Validation Data Gallery
Product Information
KE10027 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MIF ELISA kit is to be used to detect and quantify protein levels of endogenous MIF. The assay recognizes mouse MIF. An antibody specific for MIF has been pre-coated onto the microwells. The MIF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse MIF is added to detect the captured mouse MIF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse MIF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 2.3 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 17319 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatants | 109% | 86%-119% |
| Mouse serum | 104% | 87%-117% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 47.1 | 2.9 | 6.2 |
| 2 | 20 | 423.9 | 16.5 | 3.8 |
| 3 | 20 | 1,663.7 | 99.9 | 6.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 40.6 | 2.7 | 6.5 |
| 2 | 24 | 391.2 | 25.0 | 6.3 |
| 3 | 24 | 1,645.0 | 107.9 | 6.5 |
Background Information
Macrophage migration inhibitory factor (MIF) is an upstream regulator of the innate and adaptive immune response that is widely expressed and that exhibits chemokine-like activities. MIF play a role in the pathogenesis of inflammatory diseases, such as atherosclerosis, rheumatoid arthritis, sepsis, asthma, and acute respiratory distress syndrome. MIF is expressed by the ischemic myocardium and that it may exhibit an overall cardioprotective role during I/R injury. MIF regulates inflammatory processes by recruiting immune cells, amplifying cytokine production, inhibiting apoptosis and counter-regulating the immunosuppressive action of glucocorticoids.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | GIF, Glif, Mif |
Publications
| Species | Sample Type | Title |
|---|---|---|
J Adv Res Obacunone targets macrophage migration inhibitory factor (MIF) to impede osteoclastogenesis and alleviate ovariectomy-induced bone loss | ||
Cell Death Discov Downregulation of macrophage migration inhibitory factor attenuates NLRP3 inflammasome mediated pyroptosis in sepsis-induced AKI. | ||
Int J Biol Sci Inhibition of IGF2BP1 attenuates renal injury and inflammation by alleviating m6A modifications and E2F1/MIF pathway | ||
FASEB J Macrophage migration inhibitory factor (MIF) inhibitor 4-IPP suppresses osteoclast formation and promotes osteoblast differentiation through the inhibition of the NF-κB signaling pathway. | ||
Cytokine Macrophage migration inhibitory factor: A noval biomarker upregulates in myasthenia gravis and correlates with disease severity and relapse |