Mouse NGAL ELISA Kit
Cat no : KE10045
Synonyms
Lcn2, Lipocalin-2, Lipocalin 2, Neutrophil gelatinase-associated lipocalin, Oncogene 24p3
Validation Data Gallery
Product Information
KE10045 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse NGAL ELISA kit is to be used to detect and quantify protein levels of endogenous mouse NGAL. The assay recognizes mouse NGAL. An antibody specific for mouse NGAL has been pre-coated onto the microwells. The mouse NGAL protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse NGAL is added to detect the captured mouse NGAL protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse NGAL ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernates, Urine 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 4.0 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16819 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 106% | 95%-120% |
| Mouse urines | 116% | 92%-131% |
| Cell culture supernates | 106% | 79%-126% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 429.5 | 33.2 | 7.7 |
| 2 | 20 | 109.1 | 4.7 | 4.3 |
| 3 | 20 | 26.6 | 2.0 | 7.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 486.3 | 40.4 | 8.3 |
| 2 | 24 | 114.7 | 6.3 | 5.5 |
| 3 | 24 | 20.5 | 1.7 | 8.5 |
Background Information
Neutrophil gelatinase-associated lipocalin (NGAL; also known as neutrophil lipocalin, lipocalin-2, siderocalin, 24p3, or LCN2) is a small molecule of almost 25 kd that belongs to the well-defined superfamily of proteins called lipocalins. NGAL initially found in activated neutrophils, in accordance with its role as an innate antibacterial factor. However, it subsequently was shown that many other types of cells, including in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Lcn2, Lipocalin-2, Lipocalin 2, Neutrophil gelatinase-associated lipocalin, Oncogene 24p3 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Phytomedicine Dihydromyricetin attenuates intracerebral hemorrhage by reversing the effect of LCN2 via the system Xc- pathway | ||
mSystems Dietary L-arabinose-induced gut dysbiosis exacerbates Salmonella infection outcome | ||
J Nanobiotechnology Autologous platelet delivery of siRNAs by autologous plasma protein self-assembled nanoparticles for the treatment of acute kidney injury | ||
J Clin Invest Immunoreceptor CD300a regulates ischemic tissue damage and adverse remodeling in the mouse heart and kidney | ||
Apoptosis GSDME-dependent astrocyte pyroptosis promotes the progression of neuroinflammation in experimental cerebral malaria. | ||
J Ethnopharmacol Yitangkang decoction in the treatment of glomerular filtration barrier damage through AMPKα1/ZDHHC8/SLC7A11/GPX4 and TGF-β/Smad signaling pathways: A multi-omics analysis. |
