Mouse VCAM-1/CD106 ELISA Kit
Cat No : KE10038
Synonyms
CD106, vascular cell adhesion molecule 1, vascular cell adhesion protein 1, Vcam 1, Vcam1
Validation Data Gallery
Product Information
KE10038 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The VCAM-1 ELISA kit is to be used to detect and quantify protein levels of endogenous VCAM-1. The assay recognizes mouse VCAM-1. An antibody specific for VCAM-1 has been pre-coated onto the microwells. The VCAM-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for VCAM-1 is added to detect the captured VCAM-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse VCAM-1/CD106 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1.5 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 22329 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 102% | 96%-118% |
| Cell culture supernatants | 92% | 85%-100% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 766.0 | 23.0 | 3.0 |
| 2 | 20 | 412.4 | 6.3 | 1.5 |
| 3 | 20 | 317.8 | 6.5 | 2.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 689.0 | 48.9 | 7.1 |
| 2 | 24 | 375.9 | 28.4 | 7.6 |
| 3 | 24 | 283.9 | 20.0 | 7.0 |
Background Information
Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106, is a transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM-1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. The VCAM-1/integrin VLA4 (α4β1) interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. VCAM-1 is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells. Soluble VCAM-1 (sVCAM-1) has been found in addition to membrane-bound VCAM-1.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD106, vascular cell adhesion molecule 1, vascular cell adhesion protein 1, Vcam 1, Vcam1 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Pharmacol Res Zedoarondiol inhibits atherosclerosis by regulating monocyte migration and adhesion via CXCL12/CXCR4 pathway. | ||
J Extracell Vesicles Endothelial cell-derived extracellular vesicles expressing surface VCAM1 promote sepsis-related acute lung injury by targeting and reprogramming monocytes | ||
Int J Biol Macromol Nattokinase attenuates endothelial inflammation through the activation of SRF and THBS1 |