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Multi-rAb™ Enhanced Anti-Rabbit/Mouse Universal Secondary System Plus

Multi-rAb™ Enhanced Anti-Rabbit/Mouse Universal Secondary System Plus is a mixture of precisely engineered recombinant monoclonal antibodies that recognize multiple complementary epitopes on rabbit/mouse IgG heavy and light chains.

Applications

IHC

Conjugate

Polymer HRP

Host

Goat

Reactivity

Rabbit,Mouse

Cat no : RGAU015

Validation Data Gallery

  • Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-4-RR (Ki67 rabbit recombinant antibody) at dilution of 1:8000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb™ Enhanced Anti-Rabbit/Mouse Universal Secondary System Plus RGAU015 was used for detection.

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-4-RR (Ki67 rabbit recombinant antibody) at dilution of 1:8000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb™ Enhanced Anti-Rabbit/Mouse Universal Secondary System Plus RGAU015 was used for detection.

  • Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 67793-1-Ig (Desmin mouse monoclonal antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb™ Enhanced Anti-Rabbit/Mouse Universal Secondary System Plus RGAU015 was used for detection.

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 67793-1-Ig (Desmin mouse monoclonal antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb™ Enhanced Anti-Rabbit/Mouse Universal Secondary System Plus RGAU015 was used for detection.

  • Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb™ Enhanced Anti-Rabbit/Mouse Universal Secondary System Plus RGAU015 was used for detection.

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb™ Enhanced Anti-Rabbit/Mouse Universal Secondary System Plus RGAU015 was used for detection.

  • Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb™ Enhanced Anti-Rabbit/Mouse Universal Secondary System Plus RGAU015 was used for detection.

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb™ Enhanced Anti-Rabbit/Mouse Universal Secondary System Plus RGAU015 was used for detection.

  • Detection principle of Multi-rAb™ Enhanced Anti-Rabbit/Mouse Universal Secondary System Plus (RGAU015). The system is an ultra sensitive system based on recombinant secondary and polymer HRP techniques, allowing 200-300 HRP molecules been brought to one primary antibody.

    Detection principle of Multi-rAb™ Enhanced Anti-Rabbit/Mouse Universal Secondary System Plus (RGAU015). The system is an ultra sensitive system based on recombinant secondary and polymer HRP techniques, allowing 200-300 HRP molecules been brought to one primary antibody.

Product Information

RGAU015 targets Mouse IgG (H+L) and Rabbit IgG (H+L) in IHC. It is highly specific to mouse IgG and rabbit IgG and shows minimal reactivity for human, pig and bovine IgGs.

Concentration Ready to use
Applications IHC
Host Species Goat
Species Reactivity Rabbit,Mouse
Physical State Liquid
Suggested Dilution Range Ready to use
Purity Protein G purification
Storage Buffer TBST+10 mg/mL BSA+1 mg/mL gelatin + 0.1% proclin300
Storage Instructions Store at 2-8°C. Stable for one year after shipment.
ClonalityMulticlonal recombinant
RRIDAB_3677418
Note1. This product is designed for staining on auto-stainers. The background may be seen by manual staining.
2. Contact us before placing the order.
3. For diagnostic purposes, please contact us.

Chinese version
注意：
1. 本产品主要适用于机染，手工染色可能会有较强背景。
2. 采购前请先联系客服。
3. 临床诊断版本请联系我们。

背景介绍

Usage:
1. After the primary antibody reaction, wash the slides with washing buffer. Apply component A from this product to the slides. React for 10-20 minutes at room temperature.
2. After the reaction, apply component B from this product to the slides. React for 10-20 minutes at room temperature.
3. Wash and develop the signal by adding substrate.

Chinese version
使用方法：
1. 一抗反应结束后，用洗涤液洗涤，向切片上滴加本产品中的A组份，室温反应10-20分钟；
2. 反应结束后用洗涤液洗涤，向切片上滴加本产品中的B组份，室温反应10-20分钟；
3. 反应结束后用洗涤液洗涤即可加底物显色。