• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

APPBP1 Polyclonal antibody

APPBP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14863-1-AP

Print datasheet

Synonyms

NAE1, HPP1, HPP 1, APP-BP1, APP BP1

验证数据展示

  • WB analysis of human kidney using 14863-1-AP

    WB analysis of human kidney using 14863-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 14863-1-AP (APPBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse kidney using 14863-1-AP

    IP experiment of mouse kidney using 14863-1-AP

    IP result of anti-APPBP1 (IP:14863-1-AP, 4ug; Detection:14863-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

  • IHC staining of mouse salivary gland using 14863-1-AP

    IHC staining of mouse salivary gland using 14863-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse salivary gland tissue slide using 14863-1-AP (APPBP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman kidney tissue, human brain tissue, mouse brain tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inmouse salivary gland tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14863-1-AP targets APPBP1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Immunogen APPBP1 fusion protein Ag6672 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NEDD8 activating enzyme E1 subunit 1
Synonyms NAE1, HPP1, HPP 1, APP-BP1, APP BP1
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC000480
Gene Symbol NAE1/APPBP1
Gene ID (NCBI) 8883
RRIDAB_2251388
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13564
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for APPBP1 antibody 14863-1-APDownload protocol
IHC protocol for APPBP1 antibody 14863-1-APDownload protocol
IP protocol for APPBP1 antibody 14863-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Proc Natl Acad Sci U S A

Polo-like kinases mediate cell survival in mitochondrial dysfunction.

Authors - Takumi Matsumoto
  • KD Validated
View Article