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验证数据展示

  • WB analysis of human kidney using 14863-1-AP

    WB analysis of human kidney using 14863-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 14863-1-AP (APPBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse kidney using 14863-1-AP

    IP experiment of mouse kidney using 14863-1-AP

    IP result of anti-APPBP1 (IP:14863-1-AP, 4ug; Detection:14863-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

  • IHC staining of mouse salivary gland using 14863-1-AP

    IHC staining of mouse salivary gland using 14863-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse salivary gland tissue slide using 14863-1-AP (APPBP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NAE1/APPBP1 antibody included
  • Featured Product
  • KD/KO验证

APPBP1 Polyclonal antibody

APPBP1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 14863-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

NAE1, HPP1, HPP 1, APP-BP1, APP BP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman kidney tissue, human brain tissue, mouse brain tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inmouse salivary gland tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NAE1/APPBP1 Ready-To-Use IHC Kit. NAE1/APPBP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14863-1-AP targets APPBP1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 APPBP1 fusion protein Ag6672 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NEDD8 activating enzyme E1 subunit 1
别名 NAE1, HPP1, HPP 1, APP-BP1, APP BP1
计算分子量 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC000480
基因名称 NAE1/APPBP1
Gene ID (NCBI) 8883
RRIDAB_2251388
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13564
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for APPBP1 antibody 14863-1-APDownload protocol
IHC protocol for APPBP1 antibody 14863-1-APDownload protocol
IP protocol for APPBP1 antibody 14863-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Proc Natl Acad Sci U S A

Polo-like kinases mediate cell survival in mitochondrial dysfunction.

Authors - Takumi Matsumoto
  • KD Validated
View Article
humanIHC

Heliyon

Dual role of targeting NAE1 in nasopharyngeal carcinoma: Antitumor effects yet inducing radiotherapy resistance

Authors - Qinsong Liu
View Article