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- KD/KO Validated
NLRP3 Polyclonal antibody
NLRP3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA and More (4)
Conjugate
Unconjugated
491
验证数据展示
经过测试的应用
Positive WB detected in | Raji cells, THP-1 cells |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | THP-1 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
19771-1-AP targets NLRP3 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP, ELISA, Cell treatment |
Tested Reactivity | human |
Cited Reactivity | human, pig, bovine, goat |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | NLR family, pyrin domain containing 3 |
Synonyms | NACHT, LRR and PYD domains-containing protein 3, EC:3.6.4.-, Cold-induced autoinflammatory syndrome 1 protein, CIAS1, C1orf7 |
Calculated Molecular Weight | 118 kDa |
Observed Molecular Weight | 110 kDa |
GenBank Accession Number | NM_001127461 |
Gene Symbol | NLRP3 |
Gene ID (NCBI) | 114548 |
RRID | AB_10646484 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q96P20 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for NLRP3 antibody 19771-1-AP | Download protocol |
IHC protocol for NLRP3 antibody 19771-1-AP | Download protocol |
IF protocol for NLRP3 antibody 19771-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation | ||
J Hazard Mater Bile acid metabolism is altered in learning and memory impairment induced by chronic lead exposure | ||
Sci Adv Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer's disease. | ||
Gut Microbes Weizmannia coagulans BCF-01: a novel gastrogenic probiotic for Helicobacter pylori infection control | ||
Nat Commun UAF1 deubiquitinase complexes facilitate NLRP3 inflammasome activation by promoting NLRP3 expression. | ||