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NANOG Polyclonal antibody
NANOG Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IF/ICC, FC (Intra), ELISA and More (2)
Conjugate
Unconjugated
239
验证数据展示
经过测试的应用
Positive WB detected in | NCCIT cells, rat brain tissue, mouse brain tissue, mouse embryo tissue |
Positive IF/ICC detected in | human embronic stem cells |
Positive FC (Intra) detected in | NCCIT cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14295-1-AP targets NANOG in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IF/ICC, FC (Intra), ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, marmoset |
Immunogen | NANOG fusion protein Ag5645 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | Nanog homeobox |
Synonyms | hNanog |
Calculated Molecular Weight | 35 kDa |
Observed Molecular Weight | 35-40 kDa |
GenBank Accession Number | BC160187 |
Gene Symbol | NANOG |
Gene ID (NCBI) | 79923 |
RRID | AB_1607719 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9H9S0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for NANOG antibody 14295-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Stem Cell A combined cell and gene therapy approach for homotopic reconstruction of midbrain dopamine pathways using human pluripotent stem cells. | ||
Cell Stem Cell Decoding the temporal and regional specification of microglia in the developing human brain. | ||
Mol Cancer Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling. | ||
Mol Cell RNA Targets Ribogenesis Factor WDR43 to Chromatin for Transcription and Pluripotency Control. | ||
Nat Commun Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice. | ||
Nat Commun TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling. |