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- KD/KO Validated
NDUFS4 Polyclonal antibody
NDUFS4 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
产品信息
15849-1-AP targets NDUFS4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Immunogen | NDUFS4 fusion protein Ag8633 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Q reductase) |
| Synonyms | AQDQ, CI 18 kDa, CI AQDQ, Complex I 18 kDa, Complex I AQDQ, NDUFS4 |
| Calculated Molecular Weight | 175 aa, 20 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC005270 |
| Gene Symbol | NDUFS4 |
| Gene ID (NCBI) | 4724 |
| RRID | AB_2878191 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43181 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NDUFS4 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4 mitochondrial), also known as AQDQ, is a member of NADH dehydrogenase (ubiquinone) iron-sulfur (NDUFS) protein family. It is a peripheral membrane protein located on the matrix side of the inner mitochondrial membrane. It is reported that the loss of NDUFS4 protein expression in immunohistochemistry (IHC) reliably diagnoses papillary thyroid carcinoma(PMID: 34792302). Also, NDUFS4 promotes tumor progression and predicts prognosis in gastric cancer(PMID: 36044738).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NDUFS4 antibody 15849-1-AP | Download protocol |
| IHC protocol for NDUFS4 antibody 15849-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Immunity Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation.
| ||
Theranostics Proteomic analysis reveals ginsenoside Rb1 attenuates myocardial ischemia/reperfusion injury through inhibiting ROS production from mitochondrial complex I. | ||
EBioMedicine SIRT5 deacylates metabolism-related proteins and attenuates hepatic steatosis in ob/ob mice. | ||
Arch Biochem Biophys Epigallocatechin-3-gallate exerts cardioprotective effects related to energy metabolism in pressure overload-induced cardiac dysfunction. |
