验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15849-1-AP targets NDUFS4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | NDUFS4 fusion protein Ag8633 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Q reductase) |
| 别名 | AQDQ, CI 18 kDa, CI AQDQ, Complex I 18 kDa, Complex I AQDQ, NDUFS4 |
| 计算分子量 | 175 aa, 20 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC005270 |
| 基因名称 | NDUFS4 |
| Gene ID (NCBI) | 4724 |
| RRID | AB_2878191 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43181 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NDUFS4 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4 mitochondrial), also known as AQDQ, is a member of NADH dehydrogenase (ubiquinone) iron-sulfur (NDUFS) protein family. It is a peripheral membrane protein located on the matrix side of the inner mitochondrial membrane. It is reported that the loss of NDUFS4 protein expression in immunohistochemistry (IHC) reliably diagnoses papillary thyroid carcinoma(PMID: 34792302). Also, NDUFS4 promotes tumor progression and predicts prognosis in gastric cancer(PMID: 36044738).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NDUFS4 antibody 15849-1-AP | Download protocol |
| IHC protocol for NDUFS4 antibody 15849-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Immunity Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation.
| ||
Theranostics Proteomic analysis reveals ginsenoside Rb1 attenuates myocardial ischemia/reperfusion injury through inhibiting ROS production from mitochondrial complex I. | ||
EBioMedicine SIRT5 deacylates metabolism-related proteins and attenuates hepatic steatosis in ob/ob mice. | ||
Arch Biochem Biophys Epigallocatechin-3-gallate exerts cardioprotective effects related to energy metabolism in pressure overload-induced cardiac dysfunction. | ||
Cell Commun Signal The dose-dependent dual effects of alpha-ketoglutarate (AKG) on cumulus oocyte complexes during in vitro maturation | ||
Sci Rep Mitochondrial complex I inhibition enhances astrocyte responsiveness to pro-inflammatory stimuli |
