验证数据展示
经过测试的应用
| Positive WB detected in | Y79 cells, mouse pancreas tissue, rat pancreas tissue |
| Positive IP detected in | Y79 cells |
| Positive IHC detected in | rat brain tissue, human pancreas cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12081-1-AP targets NEUROD1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | NEUROD1 fusion protein Ag2713 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | neurogenic differentiation 1 |
| 别名 | NEUROD, Class A basic helix-loop-helix protein 3, bHLHa3 |
| 计算分子量 | 356 aa, 40 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC009046 |
| 基因名称 | NEUROD1 |
| Gene ID (NCBI) | 4760 |
| RRID | AB_2877823 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13562 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NeuroD is a member of the basic helix-loop-helix (bHLH) family of transcription factors. The basic helix-loop-helix (bHLH) proteins are transcription factors that are required for several aspects of development, including cell type determination, terminal differentiation and sex determination. Members of the myogenic determination family, MyoD, myf5, myogenin and MRF4, all have bHLH domains.These proteins function by forming heterodimers with E-proteins and binding to the canonical E-box sequence CANNTG. Neuro D is expressed transiently in a subset of neurons in the central and peripheral nervous systems at the time of their terminal differentiation into mature neurons. Moreover, ectopic expression of Neuro D in Xenopus embryos induces premature differentiation of neuronal precursors and Neuro D can convert presumptive epidermal cells into neurons.The lack of NeuroD in the brain results in severe defects in development. Human mutations have been linked to a number of types of diabetes including type I diabetes mellitus and maturity-onset diabetes of the young. The calculated molecular weight of NEUROD1 is 39 kDa, but the modified NEUROD1 protein is about 45-50 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NEUROD1 antibody 12081-1-AP | Download protocol |
| IHC protocol for NEUROD1 antibody 12081-1-AP | Download protocol |
| IF protocol for NEUROD1 antibody 12081-1-AP | Download protocol |
| IP protocol for NEUROD1 antibody 12081-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Prototypical oncogene family Myc defines unappreciated distinct lineage states of small cell lung cancer. | ||
Sci Adv Neurons burdened by DNA double-strand breaks incite microglia activation through antiviral-like signaling in neurodegeneration | ||
Biomaterials Three-dimensional cell-culture platform based on hydrogel with tunable microenvironmental properties to improve insulin-secreting function of MIN6 cells. | ||
Proc Natl Acad Sci U S A CHD8 dosage regulates transcription in pluripotency and early murine neural differentiation. | ||
