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验证数据展示

  • IHC staining of rat brain using 60189-1-Ig (same clone as 60189-1-PBS)

    IHC staining of rat brain using 60189-1-Ig (same clone as 60189-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60189-1-Ig (NF-L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60189-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NF-L antibody included
  • WB analysis using 60189-1-Ig (same clone as 60189-1-PBS)

    WB analysis using 60189-1-Ig (same clone as 60189-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60189-1-Ig (NF-L antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60189-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 60189-1-Ig (same clone as 60189-1-PBS)

    IF Staining of mouse brain using 60189-1-Ig (same clone as 60189-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 60189-1-Ig (NF-L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60189-1-PBS in a different storage buffer formulation.

  • FC experiment of PC-12 using 60189-1-Ig (same clone as 60189-1-PBS)

    FC experiment of PC-12 using 60189-1-Ig (same clone as 60189-1-PBS)

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-L (60189-1-Ig, Clone:5C12G4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60189-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 60189-1-Ig (same clone as 60189-1-PBS)

    IHC staining of rat brain using 60189-1-Ig (same clone as 60189-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60189-1-Ig (NF-L antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60189-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NF-L antibody included

NF-L Monoclonal antibody, PBS Only

NF-L Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 60189-1-PBS

Print datasheet

CloneNo. 5C12G4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Synonyms

NEFL, NFL, NF68, neurofilament, 5C12G4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NF-L Ready-To-Use IHC Kit. NF-L primary antibody included.

产品信息

60189-1-PBS targets NF-L in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF-P, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen NF-L fusion protein Ag15178 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name neurofilament, light polypeptide
Synonyms NEFL, NFL, NF68, neurofilament, 5C12G4
Calculated Molecular Weight 543 aa, 62 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC039237
Gene Symbol NF-L
Gene ID (NCBI) 4747
RRIDAB_10860271
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07196
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa.This antibody is specific to NEFL.