IkB Alpha Polyclonal antibody
IkB Alpha Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | PC-3 cells, HeLa cells, Jurkat cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human cervical cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
发表文章中的应用
WB | See 10 publications below |
产品信息
51066-1-AP targets IkB Alpha in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IHC, IP, WB, ELISA |
Cited Applications | WB |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Immunogen | Peptide |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha |
Synonyms | I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA |
Calculated molecular weight | 36 kDa |
Observed molecular weight | 39 kDa |
GenBank accession number | BC002601 |
Gene symbol | NFKBIA |
Uniprot | P25963 |
Gene ID (NCBI) | 4792 |
RRID | AB_2235946 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.
实验方案
Product Specific Protocols | |
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WB protocol for IkB Alpha antibody 51066-1-AP | Download protocol |
IHC protocol for IkB Alpha antibody 51066-1-AP | Download protocol |
IP protocol for IkB Alpha antibody 51066-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Hazard Mater Melatonin alleviates Ochratoxin A-induced liver inflammation involved intestinal microbiota homeostasis and microbiota-independent manner. | ||
J Immunol Enterovirus 71 2C protein inhibits TNF-α-mediated activation of NF-κB by suppressing IκB kinase β phosphorylation. | ||
Front Immunol Aspartate Metabolism Facilitates IL-1β Production in Inflammatory Macrophages. | ||
Nutrients Fish Hydrolysate Supplementation Containing n-3 Long Chain Polyunsaturated Fatty Acids and Peptides Prevents LPS-Induced Neuroinflammation. | ||