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验证数据展示

  • WB analysis of HEK-293 using 10174-1-AP

    WB analysis of HEK-293 using 10174-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10174-1-AP (NKIRAS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 10174-1-AP

    IP experiment of HEK-293 using 10174-1-AP

    IP result of anti-NKIRAS2 (IP:10174-1-AP, 4ug; Detection:10174-1-AP 1:300) with HEK-293 cells lysate 2400 ug.

  • IF Staining of A431 using 10174-1-AP

    IF Staining of A431 using 10174-1-AP

    Immunofluorescent analysis of A431 cells using 10174-1-AP (NKIRAS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

NKIRAS2 Polyclonal antibody

NKIRAS2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 10174-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

KBRAS2, KappaB-Ras2, kappaB Ras2, Kappa B-Ras protein 2, Kappa B Ras protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10174-1-AP targets NKIRAS2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 NKIRAS2 fusion protein Ag0221 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NFKB inhibitor interacting Ras-like 2
别名 KBRAS2, KappaB-Ras2, kappaB Ras2, Kappa B-Ras protein 2, Kappa B Ras protein 2
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC007450
基因名称 NKIRAS2
Gene ID (NCBI) 28511
RRIDAB_2298444
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NYR9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

I-Kappa-B-interacting Ras-like protein 2 (KBRAS2, MGC:4553), interact with the PEST domains of IkappaBalpha and IkappaBbeta [inhibitors of the transcription factor nuclear factor kappa B (NF-kappaB)] and decrease their rate of degradation. KBRAS2 differs from other Ras proteins in containing Ala and Leu residues at positions 12 and 61 respectively, which are similar to those present in the oncogenic forms of Ras.

实验方案

Product Specific Protocols
WB protocol for NKIRAS2 antibody 10174-1-APDownload protocol
IF protocol for NKIRAS2 antibody 10174-1-APDownload protocol
IP protocol for NKIRAS2 antibody 10174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

κB-Ras and Ral GTPases regulate acinar to ductal metaplasia during pancreatic adenocarcinoma development and pancreatitis.

Authors - Stephanie Beel
View Article