验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HepG2 cells |
Positive IP detected in | HEK-293 cells |
Positive IF/ICC detected in | A431 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
10174-1-AP targets NKIRAS2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | NKIRAS2 fusion protein Ag0221 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | NFKB inhibitor interacting Ras-like 2 |
Synonyms | KBRAS2, KappaB-Ras2, kappaB Ras2, Kappa B-Ras protein 2, Kappa B Ras protein 2 |
Calculated Molecular Weight | 22 kDa |
Observed Molecular Weight | 22 kDa |
GenBank Accession Number | BC007450 |
Gene Symbol | NKIRAS2 |
Gene ID (NCBI) | 28511 |
RRID | AB_2298444 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9NYR9 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
I-Kappa-B-interacting Ras-like protein 2 (KBRAS2, MGC:4553), interact with the PEST domains of IkappaBalpha and IkappaBbeta [inhibitors of the transcription factor nuclear factor kappa B (NF-kappaB)] and decrease their rate of degradation. KBRAS2 differs from other Ras proteins in containing Ala and Leu residues at positions 12 and 61 respectively, which are similar to those present in the oncogenic forms of Ras.
实验方案
Product Specific Protocols | |
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WB protocol for NKIRAS2 antibody 10174-1-AP | Download protocol |
IF protocol for NKIRAS2 antibody 10174-1-AP | Download protocol |
IP protocol for NKIRAS2 antibody 10174-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |