NKX3-1 Polyclonal antibody

NKX3-1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13069-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

BAPX2, Homeobox protein Nkx 3.1, NK3 homeobox 1, NKX3, NKX3 1, NKX3.1, NKX3-1, NKX3A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inLNCaP cells, human testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NKX3.1 Ready-To-Use IHC Kit. NKX3.1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 2 publications below

产品信息

13069-1-AP targets NKX3-1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 NKX3-1 fusion protein Ag4070 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NK3 homeobox 1
别名 BAPX2, Homeobox protein Nkx 3.1, NK3 homeobox 1, NKX3, NKX3 1, NKX3.1, NKX3-1, NKX3A
计算分子量 234 aa, 26 kDa
观测分子量34-38 kDa
GenBank蛋白编号BC074864
基因名称 NKX3-1
Gene ID (NCBI) 4824
RRIDAB_10640580
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99801
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NKX3-1, also named as NKX3.1 and NKX3A, belongs to the NK-3 homeobox family. It is a transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. NKX3-1 plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. It act as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells(PMID:19462257). The calcualted molecular weight of NKX3-1 is 26 kDa, but modified NKX3-1 is about 32-35 kDa. (PMID: 18597829)

实验方案

Product Specific Protocols
WB protocol for NKX3-1 antibody 13069-1-APDownload protocol
IHC protocol for NKX3-1 antibody 13069-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

bioRxiv

ERG activates a stem-like proliferation-differentiation program in prostate epithelial cells with mixed basal-luminal identity

Authors - Weiran Feng
mouseIHC

Sci Rep

Prostatic androgen receptor signaling shows an age-related and lobe-specific alteration in mice

Authors - Han Zhang
Loading...