验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, human testis tissue |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 2 publications below |
产品信息
13069-1-AP targets NKX3-1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | IHC |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | NKX3-1 fusion protein Ag4070 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | NK3 homeobox 1 |
别名 | BAPX2, Homeobox protein Nkx 3.1, NK3 homeobox 1, NKX3, NKX3 1, NKX3.1, NKX3-1, NKX3A |
计算分子量 | 234 aa, 26 kDa |
观测分子量 | 34-38 kDa |
GenBank蛋白编号 | BC074864 |
基因名称 | NKX3-1 |
Gene ID (NCBI) | 4824 |
RRID | AB_10640580 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q99801 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NKX3-1, also named as NKX3.1 and NKX3A, belongs to the NK-3 homeobox family. It is a transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. NKX3-1 plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. It act as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells(PMID:19462257). The calcualted molecular weight of NKX3-1 is 26 kDa, but modified NKX3-1 is about 32-35 kDa. (PMID: 18597829)
实验方案
Product Specific Protocols | |
---|---|
WB protocol for NKX3-1 antibody 13069-1-AP | Download protocol |
IHC protocol for NKX3-1 antibody 13069-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
bioRxiv ERG activates a stem-like proliferation-differentiation program in prostate epithelial cells with mixed basal-luminal identity | ||
Sci Rep Prostatic androgen receptor signaling shows an age-related and lobe-specific alteration in mice |