验证数据展示
经过测试的应用
| Positive WB detected in | U-87 MG cells, HEK-293T cells |
| Positive IP detected in | HEK-293 cells, U-87 MG cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28580-1-AP targets NOTCH2 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, CoIP |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | NOTCH2 fusion protein Ag29413 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | Notch homolog 2 (Drosophila) |
| Synonyms | Notch homolog 2 (Drosophila), Notch 2, N2ICD, N2ECD, hN2 |
| Calculated Molecular Weight | 265 kDa |
| Observed Molecular Weight | 110 kDa, 265 kDa |
| GenBank Accession Number | NM_024408 |
| Gene Symbol | NOTCH2 |
| Gene ID (NCBI) | 4853 |
| RRID | AB_2881175 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q04721 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NOTCH2 (Neurogenic locus notch homolog protein 2) belongs to the NOTCH family.. In kidney, previous reports demonstrated that Notch1 and Notch2 were activated in mammalian nephrogenesis (PMID: 24526233). Notch2 is expressed in many cell types of most lineages in the hematolymphoid compartment and has specific roles in differentiation and function of various immune cells. Notch2 is required for development of splenic marginal zone B cells and regulates differentiation of dendritic cells (DCs) in the spleen. Notch2 appears to play some specific roles in the intestinal immunity, given that the fate of mast cells and a subset of DCs is regulated by Notch2 in the intestine. Notch2 also has important roles in helper T cell divergence from naïve CD4 T cells and activation of cytotoxic T cells (PMID: 22695918).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NOTCH2 antibody 28580-1-AP | Download protocol |
| IHC protocol for NOTCH2 antibody 28580-1-AP | Download protocol |
| IP protocol for NOTCH2 antibody 28580-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Lett Therapeutic targeting m6A-guided miR-146a-5p signaling contributes to the melittin-induced selective suppression of bladder cancer. | ||
Life Sci GALNT2 promotes cell proliferation, migration, and invasion by activating the Notch/Hes1-PTEN-PI3K/Akt signaling pathway in lung adenocarcinoma. | ||
Ann Transl Med SLC6A8 is involved in the progression of non-small cell lung cancer through the Notch signaling pathway. | ||
Transl Androl Urol Evaluation of NOTCH family genes' expression and prognostic value in prostate cancer. | ||
Front Oncol A comprehensive role evaluation and mechanism exploration of POGLUT2 in pan-cancer |