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  • Featured Product
  • KD/KO Validated

NSE Monoclonal antibody, PBS Only

NSE Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6F8G3

Cat no : 66150-1-PBS

Print datasheet

Synonyms

ENO2, Gamma enolase, Enolase 2, EC:4.2.1.11, 6F8G3

验证数据展示

  • WB analysis using 66150-1-Ig (same clone as 66150-1-PBS)

    WB analysis using 66150-1-Ig (same clone as 66150-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 66150-1-Ig (same clone as 66150-1-PBS)

    FC experiment of SH-SY5Y using 66150-1-Ig (same clone as 66150-1-PBS)

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NSE (66150-1-Ig, Clone:6F8G3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

  • WB analysis of U-251 using 66150-1-Ig (same clone as 66150-1-PBS)

    WB analysis of U-251 using 66150-1-Ig (same clone as 66150-1-PBS)

    WB result of NSE antibody (66150-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSE transfected U-251 cells. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66150-1-Ig (same clone as 66150-1-PBS)

    IHC staining of mouse brain using 66150-1-Ig (same clone as 66150-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66150-1-Ig (same clone as 66150-1-PBS)

    IHC staining of mouse brain using 66150-1-Ig (same clone as 66150-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 66150-1-Ig (same clone as 66150-1-PBS)

    IF Staining of mouse brain using 66150-1-Ig (same clone as 66150-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NSE antibody (66150-1-Ig, Clone: 6F8G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 66150-1-Ig (same clone as 66150-1-PBS)

    IF Staining of mouse brain using 66150-1-Ig (same clone as 66150-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NSE antibody (66150-1-Ig, Clone: 6F8G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

产品信息

66150-1-PBS targets NSE in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF-P, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen NSE fusion protein Ag19106 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name enolase 2 (gamma, neuronal)
Synonyms ENO2, Gamma enolase, Enolase 2, EC:4.2.1.11, 6F8G3
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC002745
Gene Symbol NSE
Gene ID (NCBI) 2026
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09104
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part.