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  • Featured Product
  • KD/KO Validated

NSUN2 Polyclonal antibody

NSUN2 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

FC, IF/ICC, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 20854-1-AP

Print datasheet

Synonyms

hTrm4, MISU, NSUN2, SAKI, TRM4

验证数据展示

  • IHC staining of human breast cancer using 20854-1-AP

    IHC staining of human breast cancer using 20854-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20854-1-AP (NSUN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NSUN2 antibody included
  • WB analysis using 20854-1-AP

    WB analysis using 20854-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20854-1-AP (NSUN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 20854-1-AP

    WB analysis using 20854-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20854-1-AP (NSUN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 20854-1-AP

    WB analysis using 20854-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20854-1-AP (NSUN2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 20854-1-AP

    WB analysis of HepG2 using 20854-1-AP

    WB result of NSUN2 antibody (20854-1-AP; 1:60000; room temperature for 1.5 hours) with sh-Control and sh-NSUN2 transfected HepG2 cells.

  • IP experiment of HeLa using 20854-1-AP

    IP experiment of HeLa using 20854-1-AP

    IP result of anti-NSUN2 (IP:20854-1-AP, 4ug; Detection:20854-1-AP 1:1000) with HeLa cells lysate 4000ug.

  • IF Staining of A549 using 20854-1-AP

    IF Staining of A549 using 20854-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using NSUN2 antibody (20854-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IF Staining of HepG2 using 20854-1-AP

    IF Staining of HepG2 using 20854-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN2 antibody (20854-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • FC experiment of HeLa using 20854-1-AP

    FC experiment of HeLa using 20854-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human NSUN2 (20854-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHT-1080 cells, HeLa cells, HepG2 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells, HepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NSUN2 Ready-To-Use IHC Kit. NSUN2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:750-1:3000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20854-1-AP targets NSUN2 in WB,IP,IHC,IF,FC,ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB,IP,IHC,IF,CoIP
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen NSUN2 fusion protein Ag14791 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NOL1/NOP2/Sun domain family, member 2
Synonyms hTrm4, MISU, NSUN2, SAKI, TRM4
Calculated Molecular Weight 767 aa, 86 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC001041
Gene Symbol NSUN2
Gene ID (NCBI) 54888
RRIDAB_10693629
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ08J23
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.

实验方案

Product Specific Protocols
WB protocol for NSUN2 antibody 20854-1-APDownload protocol
IHC protocol for NSUN2 antibody 20854-1-APDownload protocol
IF protocol for NSUN2 antibody 20854-1-APDownload protocol
IP protocol for NSUN2 antibody 20854-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Signal Transduct Target Ther

tRF-Gln-CTG-026 ameliorates liver injury by alleviating global protein synthesis

Authors - Sunyang Ying
View Article
humanWB

Nat Biotechnol

Identification of direct targets and modified bases of RNA cytosine methyltransferases.

Authors - Khoddami Vahid V
View Article
mouseWB

Cell

The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.

Authors - Deniz Simsek
View Article
humanWB,IHC

Nat Cell Biol

5-methylcytosine promotes pathogenesis of bladder cancer through stabilizing mRNAs.

Authors - Xin Chen
View Article
humanWB

Nat Protoc

Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP.

Authors - Vahid Khoddami
View Article
humanWB,IF

Cell Host Microbe

Epitranscriptomic Addition of m5C to HIV-1 Transcripts Regulates Viral Gene Expression.

Authors - David G Courtney
  • KO Validated
View Article