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  • KD/KO Validated

NSUN5 Polyclonal antibody

NSUN5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 15449-1-AP

Print datasheet

Synonyms

NSUN5A, NOL1-related protein, NOL1R, NOL1, EC:2.1.1.-



经过测试的应用

Positive WB detected inHeLa cells, A549 cells, human kidney tissue, human placenta tissue
Positive IHC detected inmouse skeletal muscle tissue, human placenta tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15449-1-AP targets NSUN5 in WB, IHC, IF, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, RIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen NSUN5 fusion protein Ag7711 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NOL1/NOP2/Sun domain family, member 5
Synonyms NSUN5A, NOL1-related protein, NOL1R, NOL1, EC:2.1.1.-
Calculated Molecular Weight 46.6 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC008084
Gene Symbol NSUN5
Gene ID (NCBI) 55695
RRIDAB_2153808
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96P11
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NSUN5 is a conserved RNA methyltransferase belonging to the Nop2/SUN domain family, which is highly conserved from archaea to eukaryotes and has close contact with RNA methylation because of their S-adenosyl methionine binding-domain. NSUN5 was a promoter in the progression of CRC mainly through cell cycle regulation (PMID: 32774740). Loss of NSUN5 induces growth phenotypes in mice and different mammalian cells, which is likely connected to the requirement of NSUN5 for ribosomal RNA modification and normal global protein translation, especially in highly proliferative cells (PMID: 31722427). Western blot analysis detected NSUN5 at an apparent molecular mass of 47 kDa.

实验方案

Product Specific Protocols
WB protocol for NSUN5 antibody 15449-1-APDownload protocol
IHC protocol for NSUN5 antibody 15449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Base-resolution m5C profiling across the mammalian transcriptome by bisulfite-free enzyme-assisted chemical labeling approach

Authors - Liang Lu
humanWB

Nat Chem Biol

Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation.

Authors - Wei Dai
  • KD Validated
mouseWB,IF

Glia

Cognitive deficits in mice lacking Nsun5, a cytosine-5 RNA methyltransferase, with impairment of oligodendrocyte precursor cells.

Authors - Tingting Zhang
mouseIF

Cells

Agenesis and Hypomyelination of Corpus Callosum in Mice Lacking Nsun5, an RNA Methyltransferase.

Authors - Zihao Yuan
  • KO Validated
humanIHC

Front Oncol

Identification of Expression Patterns and Potential Prognostic Significance of m5C-Related Regulators in Head and Neck Squamous Cell Carcinoma.

Authors - Zhenyuan Han
mouseWB,IF

Mol Brain

Expression of the RNA methyltransferase Nsun5 is essential for developing cerebral cortex.

Authors - Peipei Chen
  • KO Validated