- Featured Product
- KD/KO Validated
NSUN5 Polyclonal antibody
NSUN5 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells, human kidney tissue, human placenta tissue |
| Positive IHC detected in | mouse skeletal muscle tissue, human placenta tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15449-1-AP targets NSUN5 in WB, IHC, IF, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF, RIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | NSUN5 fusion protein Ag7711 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | NOL1/NOP2/Sun domain family, member 5 |
| Synonyms | NOL1, NOL1 related protein, NOL1R, NSUN5, NSUN5A, p120, WBSCR20, WBSCR20A, Ynl022cL |
| Calculated Molecular Weight | 46.6 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC008084 |
| Gene Symbol | NSUN5 |
| Gene ID (NCBI) | 55695 |
| RRID | AB_2153808 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96P11 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NSUN5 antibody 15449-1-AP | Download protocol |
| IHC protocol for NSUN5 antibody 15449-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Base-resolution m5C profiling across the mammalian transcriptome by bisulfite-free enzyme-assisted chemical labeling approach | ||
Nat Chem Biol Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation.
| ||
Glia Cognitive deficits in mice lacking Nsun5, a cytosine-5 RNA methyltransferase, with impairment of oligodendrocyte precursor cells. | ||
Cells Agenesis and Hypomyelination of Corpus Callosum in Mice Lacking Nsun5, an RNA Methyltransferase.
| ||
Front Oncol Identification of Expression Patterns and Potential Prognostic Significance of m5C-Related Regulators in Head and Neck Squamous Cell Carcinoma. | ||
Mol Brain Expression of the RNA methyltransferase Nsun5 is essential for developing cerebral cortex.
|
