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  • Featured Product
  • KD/KO Validated

NUDT21 Monoclonal antibody, PBS Only

NUDT21 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C4C8

Cat no : 66335-1-PBS

Print datasheet

Synonyms

Nucleoside diphosphate-linked moiety X motif 21, CPSF5, CPSF25, Cleavage factor Im complex 25 kDa subunit, CFIm25

验证数据展示

  • IF Staining of HeLa using 66335-1-Ig (same clone as 66335-1-PBS)

    IF Staining of HeLa using 66335-1-Ig (same clone as 66335-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUDT21 antibody (66335-1-Ig, Clone: 1C4C8 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 66335-1-Ig (same clone as 66335-1-PBS)

    WB analysis of NIH/3T3 using 66335-1-Ig (same clone as 66335-1-PBS)

    WB result of NUDT21 antibody (66335-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUDT21 transfected NIH/3T3 cells. This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66335-1-Ig (same clone as 66335-1-PBS)

    IHC staining of mouse brain using 66335-1-Ig (same clone as 66335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUDT21 antibody included
  • WB analysis of NIH/3T3 using 66335-1-Ig (same clone as 66335-1-PBS)

    WB analysis of NIH/3T3 using 66335-1-Ig (same clone as 66335-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66335-1-Ig (NUDT21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66335-1-Ig (same clone as 66335-1-PBS)

    IF Staining of HeLa using 66335-1-Ig (same clone as 66335-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUDT21 antibody (66335-1-Ig, Clone: 1C4C8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 66335-1-Ig (same clone as 66335-1-PBS)

    IHC staining of human brain using 66335-1-Ig (same clone as 66335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUDT21 antibody included
  • IF Staining of HepG2 using 66335-1-Ig (same clone as 66335-1-PBS)

    IF Staining of HepG2 using 66335-1-Ig (same clone as 66335-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66335-1-Ig(NUDT21 antibody) at dilution of 1:400 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy NUDT21 Ready-To-Use IHC Kit. NUDT21 primary antibody included.

产品信息

66335-1-PBS targets NUDT21 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen NUDT21 fusion protein Ag12260 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 21
Synonyms Nucleoside diphosphate-linked moiety X motif 21, CPSF5, CPSF25, Cleavage factor Im complex 25 kDa subunit, CFIm25
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC001403
Gene Symbol NUDT21
Gene ID (NCBI) 11051
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43809
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cleavage and polyadenylation specific factor 5, 25 kDa (CPSF5, synonym: CFIM25) is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of CPSF5 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors.