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验证数据展示

  • IF Staining of HeLa using 66335-1-Ig (same clone as 66335-1-PBS)

    IF Staining of HeLa using 66335-1-Ig (same clone as 66335-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUDT21 antibody (66335-1-Ig, Clone: 1C4C8 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 66335-1-Ig (same clone as 66335-1-PBS)

    WB analysis of NIH/3T3 using 66335-1-Ig (same clone as 66335-1-PBS)

    WB result of NUDT21 antibody (66335-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUDT21 transfected NIH/3T3 cells. This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66335-1-Ig (same clone as 66335-1-PBS)

    IHC staining of mouse brain using 66335-1-Ig (same clone as 66335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUDT21 antibody included
  • WB analysis of NIH/3T3 using 66335-1-Ig (same clone as 66335-1-PBS)

    WB analysis of NIH/3T3 using 66335-1-Ig (same clone as 66335-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66335-1-Ig (NUDT21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66335-1-Ig (same clone as 66335-1-PBS)

    IF Staining of HeLa using 66335-1-Ig (same clone as 66335-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUDT21 antibody (66335-1-Ig, Clone: 1C4C8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 66335-1-Ig (same clone as 66335-1-PBS)

    IHC staining of human brain using 66335-1-Ig (same clone as 66335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUDT21 antibody included
  • IF Staining of HepG2 using 66335-1-Ig (same clone as 66335-1-PBS)

    IF Staining of HepG2 using 66335-1-Ig (same clone as 66335-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66335-1-Ig(NUDT21 antibody) at dilution of 1:400 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

NUDT21 Monoclonal antibody, PBS Only

NUDT21 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66335-1-PBS

产品说明书

CloneNo. 1C4C8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Nucleoside diphosphate-linked moiety X motif 21, CPSF5, CPSF25, Cleavage factor Im complex 25 kDa subunit, CFIm25

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NUDT21 Ready-To-Use IHC Kit. NUDT21 primary antibody included.

产品信息

66335-1-PBS targets NUDT21 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 NUDT21 fusion protein Ag12260 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nudix (nucleoside diphosphate linked moiety X)-type motif 21
别名 Nucleoside diphosphate-linked moiety X motif 21, CPSF5, CPSF25, Cleavage factor Im complex 25 kDa subunit, CFIm25
计算分子量 26 kDa
观测分子量 27 kDa
GenBank蛋白编号BC001403
基因名称 NUDT21
Gene ID (NCBI) 11051
RRIDAB_2881715
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO43809
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cleavage and polyadenylation specific factor 5, 25 kDa (CPSF5, synonym: CFIM25) is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of CPSF5 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors.