验证数据展示
发表文章中的应用
| WB | See 1 publications below |
产品信息
69518-1-Ig targets IL-10 in WB, ELISA, Non-Neutralization applications and shows reactivity with Human samples.
| Tested Applications | ELISA, Non-Neutralization Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human |
| Cited Reactivity | mouse |
| Immunogen | human Humankine IL-10 protein HZ-1145 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | interleukin 10 |
| Synonyms | CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF |
| Gene Symbol | IL-10 |
| Gene ID (NCBI) | 3586 |
| RRID | AB_2918852 |
| Conjugate | Unconjugated |
| Form | Lyophilized Powder |
| Purification Method | Protein G purification |
| Storage Buffer | Sterile PBS. |
| Endotoxin | <0.1 EU/μg |
| Reconstitution | This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use. |
| Stability and Storage | Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products. |
背景介绍
The human IL10 gene is 4.7 kb in size and is located on the long arm of chromosome 1. It contains five exons. Biologically functional IL-10 exists in the form of a 36 kDa homodimer composed of two non-covalently bonded mono-mers each with 160 amino acids length. IL-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
This antibody is a neutralizing control antibody for IL-10, the immunogen is the same as Neutrakine 69018-1-Ig but could not neutralize human IL-10.