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NeutraKine® EPO Monoclonal antibody

NeutraKine® EPO Monoclonal Antibody for ELISA, Neutralization

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

ELISA, Neutralization

Conjugate

Unconjugated

CloneNo.

1D2H2

Cat no : 69019-1-Ig

Print datasheet

Synonyms

EP, EPO, Epoetin, erythropoietin, MVCD2, NeutraKine® EPO

验证数据展示

  • Neutralization experiment of NeutraKine® EPO using 69019-1-Ig

    Neutralization experiment of NeutraKine® EPO using 69019-1-Ig

    Recombinant human EPO (Cat.NO. HZ-1168) stimulates TF-1 cells (human erythroleukemic cell line) in a dose-dependent nammer (blue curve, refer to bottom X-left Y). The activity of human EPO (6 ng/mL HZ-1168) was neutralized by mouse anti-human EPO monoclonal antibody 69019-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 2-5 μg/mL.

  • Neutralization experiment of NeutraKine® EPO using 69019-1-Ig

    Neutralization experiment of NeutraKine® EPO using 69019-1-Ig

    Recombinant human EPO (Cat.NO. HZ-1168) stimulates TF-1 cells (human erythroleukemic cell line) in a dose-dependent nammer (blue curve, refer to bottom X-left Y). The activity of human EPO (1 ng/mL HZ-1168) was neutralized by mouse anti-human EPO monoclonal antibody 69019-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 2-5 μg/mL. The NeutraControl mouse anti-human EPO monoclonal antibody 69519-1-Ig could recognize human EPO, but could not neutralize human EPO in the range of 0.019-80 μg/mL(green curve, refer to top X-right Y).

  • ELISA experiment of Recombinant protein using 69019-1-Ig

    ELISA experiment of Recombinant protein using 69019-1-Ig

    Indirect ELISA was carried out by coating recombinant Human EPO (Cat.NO. HZ-1168) at 70 ng/well followed by blocking and adding serial diluted EPO antibody 69019-1-Ig and 69519-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

Neutralization

产品信息

69019-1-Ig targets NeutraKine® EPO in ELISA, Neutralization applications and shows reactivity with Human samples.

Tested Applications ELISA, Neutralization Application Description
Tested Reactivity Human
Immunogen human Humankine EPO protein HZ-1168 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name erythropoietin
Synonyms EP, EPO, Epoetin, erythropoietin, MVCD2, NeutraKine® EPO
Gene Symbol EPO/Erythropoietin
Gene ID (NCBI) 2056
RRIDAB_2918839
Conjugate Unconjugated
Form Lyophilized Powder
Purification MethodProtein G purification
Storage Buffer Sterile PBS.
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

背景介绍

Erythropoietin (Epo) is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. Its effect is realized by binding erythropoietin receptor (EpoR) expressed on erythroid progenitor cells. EpoR, is a glycoprotein expressed on megakaryocytes, erythroid progenitors and endothelial cells. Epo also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.

This antibody can be used to neutralize the bioactivity of EPO.