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验证数据展示

  • Neutralization experiment of NeutraKine® GM-CSF using 69003-1-Ig (same clone as 69003-1-PBS)

    Neutralization experiment of NeutraKine® GM-CSF using 69003-1-Ig (same clone as 69003-1-PBS)

    Recombinant human GM-CSF (Cat.NO. HZ-1002) stimulates proliferation of TF-1 cell line (human erythroleukemic cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human GM-CSF (0.2 ng/mL HZ-1002) is neutralized by mouse anti-human GM-CSF monoclonal antibody 69003-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 1.5-4 μg/mL This data was developed using the same antibody clone with 69003-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50067-1

    Sandwich ELISA standard curve of MP50067-1

    Sandwich ELISA standard curve of MP50067-1, GM-CSF Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 69003-1-PBS. Detection antibody: 68702-1-PBS. Standard: Eg0189. Range: 7.8-500 pg/mL.

NeutraKine® GM-CSF Monoclonal antibody, PBS Only (Capture)

NeutraKine® GM-CSF Monoclonal Antibody for Neutralization, Sandwich ELISA, Indirect ELISA
Cat No. 69003-1-PBS

产品说明书

CloneNo. 1E10E7

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

Neutralization, Sandwich ELISA, Indirect ELISA

别名

CSF2, GM-CSF, CSF 2, CSF, Colony-stimulating factor

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

69003-1-PBS targets NeutraKine® GM-CSF as part of a matched antibody pair:

MP50067-1: 69003-1-PBS capture and 68702-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 Neutralization, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human
免疫原 human Humankine GM-CSF protein HZ-1002 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 colony stimulating factor 2 (granulocyte-macrophage)
别名 CSF2, GM-CSF, CSF 2, CSF, Colony-stimulating factor
基因名称 GM-CSF
Gene ID (NCBI) 1437
RRIDAB_2882921
偶联类型 Unconjugated
形式 Lyophilized Powder
纯化方式Protein A purification
储存缓冲液 PBS only , pH 7.3
内毒素<0.1 EU/μg
复溶This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
稳定性与储存Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

背景介绍

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).

This antibody can be used to neutralize the bioactivity of GM-CSF.