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NeutraKine® IL-6 Monoclonal antibody, PBS Only

NeutraKine® IL-6 Monoclonal Antibody for IHC, ELISA, Neutralization

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IHC, ELISA, Neutralization

Conjugate

Unconjugated

CloneNo.

4G3E6

Cat no : 69001-1-PBS

Print datasheet

Synonyms

B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IFN beta 2, IFNB2, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6, NeutraKine® IL-6

验证数据展示

  • Neutralization experiment of NeutraKine® IL-6 using 69001-1-Ig (same clone as 69001-1-PBS)

    Neutralization experiment of NeutraKine® IL-6 using 69001-1-Ig (same clone as 69001-1-PBS)

    Recombinant human IL-6 (Cat.NO. HZ-1019) stimulates proliferation of hybridoma (Proteintech mouse anti-GST clone 3G12B10) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-6 (1 ng/mL HZ-1019) is neutralized by mouse anti-human IL-6 monoclonal antibody 69001-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 10-30 ng/mL. This data was developed using the same antibody clone with 69001-1-PBS in a different storage buffer formulation.

  • Neutralization experiment of NeutraKine® IL-6 using 69001-1-Ig (same clone as 69001-1-PBS)

    Neutralization experiment of NeutraKine® IL-6 using 69001-1-Ig (same clone as 69001-1-PBS)

    Recombinant human IL-6 (Cat.NO. HZ-1019) stimulates proliferation of hybridoma (Proteintech mouse anti-GST clone 3G12B10) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-6 (1 ng/mL HZ-1019) is neutralized by mouse anti-human IL-6 monoclonal antibody 69001-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 3-15 ng/mL. The NeutraControl mouse anti-human IL-6 monoclonal antibody 69501-1-Ig could recognize human IL-6 but could not neutralize human IL-6 activity in the range of 0.06 ng/mL-1000 ng/mL (green curve, refer to top X-right Y axis). This data was developed using the same antibody clone with 69001-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 69001-1-Ig (same clone as 69001-1-PBS)

    IHC staining of human tonsillitis using 69001-1-Ig (same clone as 69001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 69001-1-Ig (NeutraKine® IL-6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 69001-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 69001-1-Ig (same clone as 69001-1-PBS)

    IHC staining of human tonsillitis using 69001-1-Ig (same clone as 69001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 69001-1-Ig (NeutraKine® IL-6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 69001-1-PBS in a different storage buffer formulation.

产品信息

69001-1-PBS targets NeutraKine® IL-6 in IHC, ELISA, Neutralization applications and shows reactivity with Human samples.

Tested Applications IHC, ELISA, Neutralization Application Description
Tested Reactivity Human
Immunogen human Humankine IL-6 protein HZ-1019 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name interleukin 6 (interferon, beta 2)
Synonyms B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IFN beta 2, IFNB2, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6, NeutraKine® IL-6
Gene Symbol IL6
Gene ID (NCBI) 3569
ENSEMBL Gene IDENSG00000136244
Conjugate Unconjugated
Form Lyophilized Powder
Purification MethodProtein A purification
Storage Buffer PBS only
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

背景介绍

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

This antibody can be used to neutralize the bioactivity of IL-6.