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验证数据展示

  • Neutralization experiment of NeutraKine® IL-6 using 69001-1-Ig (same clone as 69001-1-PBS)

    Neutralization experiment of NeutraKine® IL-6 using 69001-1-Ig (same clone as 69001-1-PBS)

    Recombinant human IL-6 (Cat.NO. HZ-1019) stimulates proliferation of hybridoma (Proteintech mouse anti-GST clone 3G12B10) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-6 (1 ng/mL HZ-1019) is neutralized by mouse anti-human IL-6 monoclonal antibody 69001-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 10-30 ng/mL. This data was developed using the same antibody clone with 69001-1-PBS in a different storage buffer formulation.

  • Neutralization experiment of NeutraKine® IL-6 using 69001-1-Ig (same clone as 69001-1-PBS)

    Neutralization experiment of NeutraKine® IL-6 using 69001-1-Ig (same clone as 69001-1-PBS)

    Recombinant human IL-6 (Cat.NO. HZ-1019) stimulates proliferation of hybridoma (Proteintech mouse anti-GST clone 3G12B10) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-6 (1 ng/mL HZ-1019) is neutralized by mouse anti-human IL-6 monoclonal antibody 69001-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 3-15 ng/mL. The NeutraControl mouse anti-human IL-6 monoclonal antibody 69501-1-Ig could recognize human IL-6 but could not neutralize human IL-6 activity in the range of 0.06 ng/mL-1000 ng/mL (green curve, refer to top X-right Y axis). This data was developed using the same antibody clone with 69001-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 69001-1-Ig (same clone as 69001-1-PBS)

    IHC staining of human tonsillitis using 69001-1-Ig (same clone as 69001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 69001-1-Ig (NeutraKine® IL-6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 69001-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 69001-1-Ig (same clone as 69001-1-PBS)

    IHC staining of human tonsillitis using 69001-1-Ig (same clone as 69001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 69001-1-Ig (NeutraKine® IL-6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 69001-1-PBS in a different storage buffer formulation.

NeutraKine® IL-6 Monoclonal antibody, PBS Only

NeutraKine® IL-6 Monoclonal Antibody for IHC, Neutralization, ELISA
Cat No. 69001-1-PBS

产品说明书

CloneNo. 4G3E6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IHC, Neutralization, ELISA

别名

IL6, IL-6, 4G3E6, B-cell stimulatory factor 2, IFN-beta-2

缓冲液配方:  PBS Only
Sterile PBS
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

69001-1-PBS targets NeutraKine® IL-6 in IHC, Neutralization, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, Neutralization, ELISA Application Description
经测试反应性 human
免疫原 human Humankine IL-6 protein HZ-1019 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 6 (interferon, beta 2)
别名 IL6, IL-6, 4G3E6, B-cell stimulatory factor 2, IFN-beta-2
基因名称 IL6
Gene ID (NCBI) 3569
ENSEMBL Gene IDENSG00000136244
RRIDAB_2882919
偶联类型 Unconjugated
纯化方式Protein G purification
储存缓冲液 PBS only, pH 7.3.
储存Store at -80°C.

背景介绍

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

This antibody can be used to neutralize the bioactivity of IL-6.