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NeutraKine®VEGF165/121 Monoclonal antibody

NeutraKine®VEGF165/121 Monoclonal Antibody for ELISA, Neutralization

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

ELISA, Neutralization

Conjugate

Unconjugated

CloneNo.

6C3D5

Cat no : 69025-1-Ig

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Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF 121, VEGF 165, VEGF A, VEGF121, VEGF165, VEGFA, VPF

验证数据展示

  • Neutralization experiment of NeutraKine®VEGF165/121 using 69025-1-Ig

    Neutralization experiment of NeutraKine®VEGF165/121 using 69025-1-Ig

    Recombinant human VEGF165 (Cat.NO. HZ-1038) stimulates proliferation of HUVEC (human umbilical vein endothelial) cell line in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human VEGF165 (10 ng/mL HZ-1038) is neutralized by mouse anti-human VEGF165/121 monoclonal antibody 69025-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 0.5-2 μg/mL. The NeutraControl mouse anti-human VEGF165 antibody 69525-1-Ig could recognize human VEGF165 but could not neutralize human VEGF165 acitivity in the range of 0.00244-40 μg/mL (green curve, refer to top X-right Y axis).

  • ELISA experiment of Recombinant protein using 69025-1-Ig

    ELISA experiment of Recombinant protein using 69025-1-Ig

    Indirect ELISA was carried out by coating recombinant Human VEGF165 (Cat.NO. HZ-1038) and VEGF121 (Cat.NO. HZ-1204) respectively at 70 ng/well followed by blocking and adding serial diluted VEGF165/121 antibody 69025-1-Ig and VEGF165 antibody 69525-1-Ig respectively. HRP-goat anti-mouse was used for detection. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. The result suggsts that 69025-1-Ig recognizes both VEGF165 and VEGF121 while 69525-1-Ig recognize VEGF165 only.

Planning an IHC experiment? We recommend our IHCeasy VEGFA/VEGF Ready-To-Use IHC Kit. VEGFA/VEGF primary antibody included.
Neutralization

发表文章中的应用

ELISASee 1 publications below

产品信息

69025-1-Ig targets NeutraKine®VEGF165/121 in ELISA, Neutralization applications and shows reactivity with Human samples.

Tested Applications ELISA, Neutralization Application Description
Cited ApplicationsELISA
Tested Reactivity Human
Cited Reactivityhuman
Immunogen human HumankineVEGF165/121 protein HZ-1038 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name vascular endothelial growth factor A
Synonyms L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF 121, VEGF 165, VEGF A, VEGF121, VEGF165, VEGFA, VPF
Gene Symbol VEGFA
Gene ID (NCBI) 7422
RRIDAB_2918845
Conjugate Unconjugated
Form Lyophilized Powder
Purification MethodProtein G purification
Storage Buffer Sterile PBS.
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

背景介绍

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ).

This antibody is used to neutralize the bioactivity of VEGF165/121. ELISA test suggests that this antibody recognize VEGF165 and VEGF121. According to sequence similarity, it may recognize other isoforms.

发表文章

SpeciesApplicationTitle
humanELISA

J Transl Med

Brevilin A is a potent anti-metastatic CRC agent that targets the VEGF-IL6-STAT3 axis in the HSCs-CRC interplay

Authors - Xueying Fan
View Article