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Nurr1/NR4A2 Monoclonal antibody, PBS Only

Nurr1/NR4A2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H5B4

Cat No : 66878-1-PBS

Print datasheet

Synonyms

HZF 3, NOT, NR4A2, NURR1, Nurr1/NR4A2, Orphan nuclear receptor NURR1, RNR1, TINUR

验证数据展示

  • WB analysis using 66878-1-Ig (same clone as 66878-1-PBS)

    WB analysis using 66878-1-Ig (same clone as 66878-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

  • WB analysis using 66878-1-Ig (same clone as 66878-1-PBS)

    WB analysis using 66878-1-Ig (same clone as 66878-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66878-1-Ig (same clone as 66878-1-PBS)

    IHC staining of human prostate cancer using 66878-1-Ig (same clone as 66878-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NR4A2 antibody included
  • IHC staining of human prostate cancer using 66878-1-Ig (same clone as 66878-1-PBS)

    IHC staining of human prostate cancer using 66878-1-Ig (same clone as 66878-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NR4A2 antibody included
Planning an IHC experiment? We recommend our IHCeasy NR4A2 Ready-To-Use IHC Kit. NR4A2 primary antibody included.

产品信息

66878-1-PBS targets Nurr1/NR4A2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse
Immunogen Nurr1/NR4A2 fusion protein Ag1418 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nuclear receptor subfamily 4, group A, member 2
Synonyms HZF 3, NOT, NR4A2, NURR1, Nurr1/NR4A2, Orphan nuclear receptor NURR1, RNR1, TINUR
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC009288
Gene Symbol NR4A2
Gene ID (NCBI) 4929
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP43354
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

NR4A2 belongs to the orphan nuclear receptor (NR) family referred to as NR4A family, which have been implicated in cell cycle regulation, apoptosis, inflammation, metabolism and more recently in carcinogenesis [PMID:22583411]. NR4A2 also has a role in the expression of several proteins that are necessary for the synthesis and regulation of DA, such as tyrosine hidroxilase, DA transporter, vesicular monoamine transporter 2, and cRET [PMID:22294735]. Act as a transcriptional regulator, NR4A2 is essential for the differentiation and maintenance of meso-diencephalic mdDA neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons [PMID: 17184956].