验证数据展示
经过测试的应用
| Positive WB detected in | IFN alpha treated A549 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human colon cancer, human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19279-1-AP targets OAS2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | OAS2 fusion protein Ag6170 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 2'-5'-oligoadenylate synthetase 2, 69/71kDa |
| 别名 | EC:2.7.7.84, 2'-5'-oligoadenylate synthase 2, 2-5A synthase 2, (2-5')oligo(A) synthase 2 |
| 计算分子量 | 82 kDa |
| 观测分子量 | 66-71 kDa |
| GenBank蛋白编号 | BC049215 |
| 基因名称 | OAS2 |
| Gene ID (NCBI) | 4939 |
| RRID | AB_10642832 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P29728 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS2, are IFN-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS2 is also named as p69 OAS / p71 OAS. It has 3 isoforms(71/69/20 kDa) produced by alternative splicing. The OAS2 isozymes are 69-71 kDa and form dimers(PMID:17765707). Glycosylation is essential for its activity(PMID:9880569). Constitutive expression of the 69-kDa form of the OAS2 protein in human cells inhibited the replication of encephalomyocarditis virus (EMCV) but not that of VSV, Sendai virus, or reovirus(PMID:16501108). This antibody is specific to isoform p71 and isoform p69 of OAS2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OAS2 antibody 19279-1-AP | Download protocol |
| IHC protocol for OAS2 antibody 19279-1-AP | Download protocol |
| IP protocol for OAS2 antibody 19279-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Redox regulation of m6A methyltransferase METTL3 in β-cells controls the innate immune response in type 1 diabetes | ||
Nat Commun Decoding non-canonical mRNA decay by the endoplasmic-reticulum stress sensor IRE1α | ||
Sci Adv IL-27 signaling activates skin cells to induce innate antiviral proteins and protects against Zika virus infection. | ||
J Invest Dermatol Skin Injury Activates A Rapid TRPV1-dependent Antiviral Protein Response. |
