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OAS2 Polyclonal antibody
OAS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | IFN alpha treated A549 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human colon cancer, human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19279-1-AP targets OAS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | OAS2 fusion protein Ag6170 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | 2'-5'-oligoadenylate synthetase 2, 69/71kDa |
| Synonyms | EC:2.7.7.84, 2'-5'-oligoadenylate synthase 2, 2-5A synthase 2, (2-5')oligo(A) synthase 2 |
| Calculated Molecular Weight | 82 kDa |
| Observed Molecular Weight | 66-71 kDa |
| GenBank Accession Number | BC049215 |
| Gene Symbol | OAS2 |
| Gene ID (NCBI) | 4939 |
| RRID | AB_10642832 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P29728 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS2, are IFN-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS2 is also named as p69 OAS / p71 OAS. It has 3 isoforms(71/69/20 kDa) produced by alternative splicing. The OAS2 isozymes are 69-71 kDa and form dimers(PMID:17765707). Glycosylation is essential for its activity(PMID:9880569). Constitutive expression of the 69-kDa form of the OAS2 protein in human cells inhibited the replication of encephalomyocarditis virus (EMCV) but not that of VSV, Sendai virus, or reovirus(PMID:16501108). This antibody is specific to isoform p71 and isoform p69 of OAS2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OAS2 antibody 19279-1-AP | Download protocol |
| IHC protocol for OAS2 antibody 19279-1-AP | Download protocol |
| IF protocol for OAS2 antibody 19279-1-AP | Download protocol |
| IP protocol for OAS2 antibody 19279-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Redox regulation of m6A methyltransferase METTL3 in β-cells controls the innate immune response in type 1 diabetes | ||
Nat Commun Decoding non-canonical mRNA decay by the endoplasmic-reticulum stress sensor IRE1α | ||
Sci Adv IL-27 signaling activates skin cells to induce innate antiviral proteins and protects against Zika virus infection. | ||
J Invest Dermatol Skin Injury Activates A Rapid TRPV1-dependent Antiviral Protein Response. |
