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  • Featured Product
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OCT4/POU5F1 Polyclonal antibody

OCT4/POU5F1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

FC, IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 11263-1-AP

Print datasheet

Synonyms

Oct 3, Oct 4, OCT3, OCT3/4, OCT4, Octamer binding protein 3, Octamer binding protein 4, OTF 3, OTF3, OTF4, POU class 5 homeobox 1, POU5F1

验证数据展示

  • WB analysis using 11263-1-AP

    WB analysis using 11263-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11263-1-AP (OCT4/POU5F1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of H7ES using 11263-1-AP

    WB analysis of H7ES using 11263-1-AP

    H7ES cells were subjected to SDS PAGE followed by western blot with 11263-1-AP (OCT4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse eye using 11263-1-AP

    IF Staining of mouse eye using 11263-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using OCT4 antibody (11263-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse eye using 11263-1-AP

    IF Staining of mouse eye using 11263-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using OCT4 antibody (11263-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 11263-1-AP

    WB analysis using 11263-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11263-1-AP (OCT4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis using 11263-1-AP

    WB analysis using 11263-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11263-1-AP (OCT4/POU5F1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 11263-1-AP

    IHC staining of human lung cancer using 11263-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11263-1-AP (OCT4/POU5F1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 11263-1-AP

    IHC staining of human lung cancer using 11263-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11263-1-AP (OCT4/POU5F1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of K-562 using 11263-1-AP

    FC experiment of K-562 using 11263-1-AP

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human OCT4/POU5F1 (11263-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 11263-1-AP

    WB analysis using 11263-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11263-1-AP (OCT4/POU5F1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293T using 11263-1-AP

    FC experiment of HEK-293T using 11263-1-AP

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human OCT4/POU5F1 (11263-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inNCCIT cells, H7ES cells, MCF-7 cells, HEK-293 cells, mouse embryo tissue, mouse brain tissue, U-251 cells, rat brain tissue, HT-29 cells, HepG2 cells, U-87 MG cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse eye tissue
Positive FC detected inK-562 cells, HEK-293T cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

产品信息

11263-1-AP targets OCT4/POU5F1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, WB, ELISA
Cited Applications FC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, pig
Immunogen OCT4/POU5F1 fusion protein Ag1794 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name POU class 5 homeobox 1
Synonyms Oct 3, Oct 4, OCT3, OCT3/4, OCT4, Octamer binding protein 3, Octamer binding protein 4, OTF 3, OTF3, OTF4, POU class 5 homeobox 1, POU5F1
Calculated molecular weight 39 kDa
Observed molecular weight50-60 kDa
GenBank accession numberBC020712
Gene symbol POU5F1
UniprotQ01860
Gene ID (NCBI) 5460
RRIDAB_2167545
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Background

Octamer-binding protein 4 (OCT4), also known as POU5F1 or OCT3/4, is a member of the POU gene family and encoded by the human gene POU5F1 (POU domain class 5 transcription factor 1). OCT4 is a member of the Octamer class of transcription factors that recognize the 8-base pair consensus motif 5'-ATGCAAAT-3'. Expression of OCT4 is associated with an undifferentiated, pluripotent stem cell phenotype and tumors. OCT4 is also expressed in the developing brain, with the highest levels found in the cortex, olfactory bulb, and the hippocampus.

 What is the molecular weight of OCT4?

The molecular weight of OCT4 is 38 kDa.

 What are the isoforms of OCT4?

The human POU51F gene consists of five exons located on chromosome 6 and can generate three mRNA isoforms through alternative splicing - OCT4A, OCT4B, and OCT4B1 (PMID: 18787205). OCT4A and OCT4B1 orchestrate gene transcription in the nucleus supporting self-renewal and pluripotency maintenance in ESCs and embryonal carcinoma cells, whereas OCT4B is localized in the cytoplasm in various non-pluripotent cell types and cannot sustain self-renewal and pluripotency.

 What is OCT4's involvement in pluripotency and self-renewal?

OCT4 forms a trimeric complex with SOX2 and DNA to control the expression of genes involved with embryonic development, such as FGF4 (PMID: 10801796), UTF1 (PMID: 10409735), or even NANOG (PMID: 15743839). OCT4 is critical for early embryogenesis (PMID: 9814708) and for embryonic stem cell pluripotency (PMID: 16153702). High levels of OCT4 are associated with the ability to self-renew, which is emphasized by OCT4 gene knockdowns promoting differentiation (PMID: 10742100). OCT4 is also one of the four key transcription factors used to generate induced pluripotent stem cells (iPSCs) by reprogramming fibroblasts to a pluripotent state (PMID: 16904174).




实验方案

Product Specific Protocols
WB protocol for OCT4/POU5F1 antibody 11263-1-APDownload protocol
IHC protocol for OCT4/POU5F1 antibody 11263-1-APDownload protocol
IF protocol for OCT4/POU5F1 antibody 11263-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Temporal proteomics during neurogenesis reveals large-scale proteome and organelle remodeling via selective autophagy

Authors - Alban Ordureau
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mouseIF

Bone Res

Type II collagen-positive progenitors are important stem cells in controlling skeletal development and vascular formation.

Authors - Xinhua Li
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humanWB

Theranostics

Carcinoma-associated fibroblasts promote the stemness and chemoresistance of colorectal cancer by transferring exosomal lncRNA H19.

Authors - Jing Ren
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humanWB

Mol Ther

Exosomal miR-1246 from glioma patient body fluids drives the differentiation and activation of myeloid-derived suppressor cells.

Authors - Wei Qiu
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human,mouseWB

Theranostics

IL20RA signaling enhances stemness and promotes the formation of an immunosuppressive microenvironment in breast cancer.

Authors - Wenjuan Gao
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humanWB

Breast Cancer Res

Targeting DCLK1 attenuates tumor stemness and evokes antitumor immunity in triple-negative breast cancer by inhibiting IL-6/STAT3 signaling

Authors - Heshu Liu
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