验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, A549 cells, mouse liver tissue, mouse pancreas tissue, ROS1728 cells, rat heart tissue, mouse lung tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells, HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy OGDH Ready-To-Use IHC Kit. OGDH primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15212-1-AP targets OGDH in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, rabbit, chicken, sheep |
| 免疫原 | OGDH fusion protein Ag7368 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) |
| 别名 | EC:1.2.4.2, E1o, Alpha-KGDH-E1, Alpha-ketoglutarate dehydrogenase, 2-oxoglutarate dehydrogenase, mitochondrial |
| 计算分子量 | 116 kDa |
| 观测分子量 | 116 kDa |
| GenBank蛋白编号 | BC004964 |
| 基因名称 | OGDH |
| Gene ID (NCBI) | 4967 |
| RRID | AB_2156759 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q02218 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing. This antibody is immunized with the C-terminal and can recognize the isoform 1 and 2 with the MW of 116 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OGDH antibody 15212-1-AP | Download protocol |
| IHC protocol for OGDH antibody 15212-1-AP | Download protocol |
| IF protocol for OGDH antibody 15212-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations. | ||
Cell Metab An Upstream Open Reading Frame in Phosphatase and Tensin Homolog Encodes a Circuit Breaker of Lactate Metabolism. | ||
Cell Res The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8. | ||
J Hepatol OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism. |
