验证数据展示

WB analysis using 10837-1-AP
various lysates were subjected to SDS PAGE followed by western blot with 10837-1-AP (OPTN antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
FC experiment of HeLa using 10837-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human OPTN (10837-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IP experiment of mouse brain using 10837-1-AP
IP result of anti-OPTN (IP:10837-1-AP, 3ug; Detection:10837-1-AP 1:800) with mouse brain tissue lysate 8000ug.
IHC staining of human gliomas using 10837-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10837-1-AP (OPTN Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human gliomas using 10837-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10837-1-AP (OPTN Antibody) at dilution of 1:200 (under 40x lens).
WB analysis of HeLa using 10837-1-AP
WB result of OPTN antibody (10837-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OPTN transfected HeLa cells.
IF Staining of rat eye using 10837-1-AP
Immunofluorescent analysis of (-20°C Acetone) fixed paraffin-embedded rat eye tissue using OPTN antibody (10837-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 10837-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using OPTN antibody (10837-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Featured Product
KD/KO Validated

OPTN/Optineurin Polyclonal antibody

OPTN/Optineurin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Cat No. 10837-1-AP

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Publications(112)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (2)

Synonyms

Optineurin, OPTN, FIP-2, FIP2, FIP 2

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

Size:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (112)
实验方案

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MSDS

经过测试的应用

Positive WB detected in	HeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	rat eye tissue
Positive IF/ICC detected in	MCF-7 cells
Positive FC (Intra) detected in	HeLa cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 14 publications below
WB	See 94 publications below
IHC	See 5 publications below
IF	See 21 publications below
IP	See 7 publications below
CoIP	See 2 publications below

产品信息

10837-1-AP targets OPTN/Optineurin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP, CoIP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, monkey, hamster
Immunogen	OPTN/Optineurin fusion protein Ag1272 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	optineurin
Synonyms	Optineurin, OPTN, FIP-2, FIP2, FIP 2
Calculated Molecular Weight	66 kDa
Observed Molecular Weight	66-70 kDa
GenBank Accession Number	BC013876
Gene Symbol	OPTN
Gene ID (NCBI)	10133
RRID	AB_2156665
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96CV9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork. OPTN has 3 isoforms with MW 66,65 and 60 kDa (refer to UniProt). Catalog#10837-1-AP recognises 66 and 70-74 kDa band, and the additional 70-74 kDa band due to phosphorylation.

实验方案

Product Specific Protocols
WB protocol for OPTN/Optineurin antibody 10837-1-AP	Download protocol
IHC protocol for OPTN/Optineurin antibody 10837-1-AP	Download protocol
IF protocol for OPTN/Optineurin antibody 10837-1-AP	Download protocol
IP protocol for OPTN/Optineurin antibody 10837-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Nature The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy. Authors - Michael Lazarou View Article
mouse	WB	Cell NF-κB Restricts Inflammasome Activation via Elimination of Damaged Mitochondria. Authors - Zhenyu Zhong View Article
human	CoIP	Mol Cell TNIP1 inhibits selective autophagy via bipartite interaction with LC3/GABARAP and TAX1BP1 Authors - François Le Guerroué View Article
human	IF	Acta Neuropathol p62 positive, TDP-43 negative, neuronal cytoplasmic and intranuclear inclusions in the cerebellum and hippocampus define the pathology of C9orf72-linked FTLD and MND/ALS. Authors - Safa Al-Sarraj View Article
human	WB	Mol Cell Loss of TAX1BP1-Directed Autophagy Results in Protein Aggregate Accumulation in the Brain. Authors - Shireen A Sarraf KO Validated View Article
human	WB	Mol Cell Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling. Authors - Susanne Zellner View Article

验证数据展示

WB analysis using 10837-1-AP

FC experiment of HeLa using 10837-1-AP

IP experiment of mouse brain using 10837-1-AP

IHC staining of human gliomas using 10837-1-AP

IHC staining of human gliomas using 10837-1-AP

WB analysis of HeLa using 10837-1-AP

IF Staining of rat eye using 10837-1-AP

IF Staining of MCF-7 using 10837-1-AP

OPTN/Optineurin Polyclonal antibody

OPTN/Optineurin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy.

NF-κB Restricts Inflammasome Activation via Elimination of Damaged Mitochondria.

TNIP1 inhibits selective autophagy via bipartite interaction with LC3/GABARAP and TAX1BP1

p62 positive, TDP-43 negative, neuronal cytoplasmic and intranuclear inclusions in the cerebellum and hippocampus define the pathology of C9orf72-linked FTLD and MND/ALS.

Loss of TAX1BP1-Directed Autophagy Results in Protein Aggregate Accumulation in the Brain.

Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.