验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, mouse brain tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | rat eye tissue |
Positive IF/ICC detected in | MCF-7 cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10837-1-AP targets OPTN/Optineurin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP, CoIP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat, pig, monkey, hamster |
免疫原 | OPTN/Optineurin fusion protein Ag1272 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | optineurin |
别名 | Optineurin, OPTN, FIP-2, FIP2, FIP 2 |
计算分子量 | 66 kDa |
观测分子量 | 66-70 kDa |
GenBank蛋白编号 | BC013876 |
基因名称 | OPTN |
Gene ID (NCBI) | 10133 |
RRID | AB_2156665 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q96CV9 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork. OPTN has 3 isoforms with MW 66,65 and 60 kDa (refer to UniProt). Catalog#10837-1-AP recognises 66 and 70-74 kDa band, and the additional 70-74 kDa band due to phosphorylation.
实验方案
Product Specific Protocols | |
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WB protocol for OPTN/Optineurin antibody 10837-1-AP | Download protocol |
IHC protocol for OPTN/Optineurin antibody 10837-1-AP | Download protocol |
IF protocol for OPTN/Optineurin antibody 10837-1-AP | Download protocol |
IP protocol for OPTN/Optineurin antibody 10837-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell TNIP1 inhibits selective autophagy via bipartite interaction with LC3/GABARAP and TAX1BP1 | ||
Acta Neuropathol p62 positive, TDP-43 negative, neuronal cytoplasmic and intranuclear inclusions in the cerebellum and hippocampus define the pathology of C9orf72-linked FTLD and MND/ALS. | ||
Mol Cell Loss of TAX1BP1-Directed Autophagy Results in Protein Aggregate Accumulation in the Brain.
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Mol Cell Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling. |