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验证数据展示

  • WB analysis of HeLa using 20112-1-AP

    WB analysis of HeLa using 20112-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20112-1-AP (OVCA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 20112-1-AP

    IF Staining of HeLa using 20112-1-AP

    Immunofluorescent analysis of HeLa cells using 20112-1-AP (OVCA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human ovary cancer using 20112-1-AP

    IHC staining of human ovary cancer using 20112-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20112-1-AP (OVCA1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

OVCA1 Polyclonal antibody

OVCA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20112-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

DPH1, DPH2L1, DPH2L, DPH1 homolog, Diphtheria toxin resistance protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20112-1-AP targets OVCA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 OVCA1 fusion protein Ag13910 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DPH1 homolog (S. cerevisiae)
别名 DPH1, DPH2L1, DPH2L, DPH1 homolog, Diphtheria toxin resistance protein 1
计算分子量 489 aa, 52 kDa
观测分子量 40-60 kDa
GenBank蛋白编号BC003099
基因名称 DPH1
Gene ID (NCBI) 1801
RRIDAB_10641038
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BZG8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OVCA1, also named as DPH1, is an evolutionarily conserved gene, indicative of a fundamental biological role in a variety of species. In humans, OVCA1 was initially mapped within the deletion region of 17p13.3 involved in early ovarian cancer (PMID: 8564979). Ovca1-null mice, mainly addressed its tumor suppressive role and potential cooperative role in modifying p53-deficient tumorigenesis (PMID: 14744934). OVCA1 acts as a component of an enzyme complex with DPH2, DPH3 and DPH4 that functionally catalyzes the first step of the diphthamide biosynthesis pathway, to posttranslationally modify a conserved histidine residue (His699 in yeast and His715 in human) of eukaryotic elongation factor 2 (eEF-2).

实验方案

Product Specific Protocols
WB protocol for OVCA1 antibody 20112-1-APDownload protocol
IHC protocol for OVCA1 antibody 20112-1-APDownload protocol
IF protocol for OVCA1 antibody 20112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols