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  • KD/KO Validated

P21 Monoclonal antibody

P21 Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

monkey and More (1)

Applications

ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

5F2F3

Cat no : 60214-1-Ig

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Synonyms

CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, p21CIP1, PIC1, SDI1, WAF1



Planning an IHC experiment? We recommend our IHCeasy CDKN1A/P21 Ready-To-Use IHC Kit. CDKN1A/P21 primary antibody included.

产品信息

60214-1-Ig targets P21 in WB, ELISA applications and shows reactivity with monkey samples.

Tested Applications ELISA Application Description
Cited ApplicationsWB
Tested Reactivity monkey
Cited Reactivityhuman
Immunogen P21 fusion protein Ag0308 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Synonyms CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, p21CIP1, PIC1, SDI1, WAF1
Calculated Molecular Weight 21 kDa
GenBank Accession NumberBC000312
Gene Symbol p21
Gene ID (NCBI) 1026
RRIDAB_11042450
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP38936
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDKN1A (p21, CIP1, WAF1), a cyclin-dependent kinase inhibitor, is necessary for proper control of the cell cycle and premature senescence. P21 is a cyclin-dependent kinase (CDK) inhibitor that suppresses proliferation by inhibiting CDK2 and CDK1 activity at the G1/S and G2/M transitions. P21 is a major mediator of p53 to induce cell cycle arrest in G1. p21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. P21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Overexpression of p21 decreases the expression levels of cell cycle progression genes and upregulates senescence-inducing genes.

实验方案

Product Specific Protocols
WB protocol for P21 antibody 60214-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer

Authors - Rong Wang
humanWB

Biomed Pharmacother

MiRNA-20a-5p promotes the growth of triple-negative breast cancer cells through targeting RUNX3.

Authors - Xiangdong Bai
humanWB

J Pathol

NFIB promotes cell survival by directly suppressing p21 transcription in p53-mutated triple-negative breast cancer.

Authors - Rong-Zong Liu
  • KD Validated
humanWB

Virulence

Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions.

Authors - Wei Jie
WB

Aging (Albany NY)

Transgenic mice overexpressing glia maturation factor-β, an oxidative stress inducible gene, show premature aging due to Zmpste24 down-regulation.

Authors - Rika Imai
humanWB

J Biol Chem

RNA binding protein RBM28 can translocate from the nucleolus to the nucleoplasm to inhibit the transcriptional activity of p53.

Authors - Xin Lin