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- KD/KO Validated
P21 Monoclonal antibody
P21 Monoclonal Antibody for ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
monkey and More (1)
Applications
ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
5F2F3
验证数据展示
Planning an IHC experiment? We recommend our IHCeasy CDKN1A/P21 Ready-To-Use IHC Kit. CDKN1A/P21 primary antibody included.
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 28 publications below |
产品信息
The immunogen of 60214-1-Ig is P21 Fusion Protein expressed in E. coli.
| Tested Applications | ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | monkey |
| Cited Reactivity | human |
| Immunogen | P21 fusion protein Ag0308 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | cyclin-dependent kinase inhibitor 1A (p21, Cip1) |
| Synonyms | CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, p21CIP1, PIC1, SDI1, WAF1 |
| Calculated Molecular Weight | 21 kDa |
| GenBank Accession Number | BC000312 |
| Gene Symbol | p21 |
| Gene ID (NCBI) | 1026 |
| RRID | AB_11042450 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P38936 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CDKN1A (p21, CIP1, WAF1), a cyclin-dependent kinase inhibitor, is necessary for proper control of the cell cycle and premature senescence. P21 is a cyclin-dependent kinase (CDK) inhibitor that suppresses proliferation by inhibiting CDK2 and CDK1 activity at the G1/S and G2/M transitions. P21 is a major mediator of p53 to induce cell cycle arrest in G1. p21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. P21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Overexpression of p21 decreases the expression levels of cell cycle progression genes and upregulates senescence-inducing genes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for P21 antibody 60214-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer | ||
Biomed Pharmacother MiRNA-20a-5p promotes the growth of triple-negative breast cancer cells through targeting RUNX3. | ||
J Pathol NFIB promotes cell survival by directly suppressing p21 transcription in p53-mutated triple-negative breast cancer.
| ||
Virulence Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions. | ||
Aging (Albany NY) Transgenic mice overexpressing glia maturation factor-β, an oxidative stress inducible gene, show premature aging due to Zmpste24 down-regulation. | ||
J Biol Chem RNA binding protein RBM28 can translocate from the nucleolus to the nucleoplasm to inhibit the transcriptional activity of p53. |
