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- KD/KO Validated
P27; KIP1 Polyclonal antibody
P27; KIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (5)
Applications
WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (2)
Conjugate
Unconjugated
204
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, NIH/3T3 cells, HepG2 cells, MCF-7 cells, Jurkat cells |
Positive IP detected in | NIH/3T3 cells |
Positive IHC detected in | human breast cancer tissue, human lung cancer tissue, human colon cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse testis tissue |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
25614-1-AP targets P27; KIP1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat, rabbit, canine, bovine, sheep |
Immunogen | P27; KIP1 fusion protein Ag22582 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | cyclin-dependent kinase inhibitor 1B (p27, Kip1) |
Synonyms | CDKN1B, P27, MEN1B, KIP1, Cyclin-dependent kinase inhibitor p27 |
Calculated Molecular Weight | 198 aa, 22 kDa |
Observed Molecular Weight | 27 kDa |
GenBank Accession Number | BC001971 |
Gene Symbol | p27 Kip1/CDKN1B |
Gene ID (NCBI) | 1027 |
RRID | AB_2880161 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P46527 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for P27; KIP1 antibody 25614-1-AP | Download protocol |
IHC protocol for P27; KIP1 antibody 25614-1-AP | Download protocol |
IF protocol for P27; KIP1 antibody 25614-1-AP | Download protocol |
IP protocol for P27; KIP1 antibody 25614-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Gastroenterology Intestinal PPARα Protects Against Colon Carcinogenesis via Regulation of Methyltransferases DNMT1 and PRMT6. | ||
Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. | ||
Mol Cancer LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer. | ||
Mol Cancer CircGPR137B/miR-4739/FTO feedback loop suppresses tumorigenesis and metastasis of hepatocellular carcinoma. | ||
Small Synergistic Silencing of Skp2 by siRNA Self-Assembled Nanoparticles as a Therapeutic Strategy for Advanced Prostate Cancer. | ||
Cell Death Dis UBE2E2 enhances Snail-mediated epithelial-mesenchymal transition and Nrf2-mediated antioxidant activity in ovarian cancer |