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P2RX4 Polyclonal antibody

P2RX4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 13534-1-AP

Print datasheet

Synonyms

ATP receptor, P2RX4, P2X purinoceptor 4, P2X4, P2X4R, Purinergic receptor



经过测试的应用

Positive WB detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13534-1-AP targets P2RX4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen P2RX4 fusion protein Ag4445 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name purinergic receptor P2X, ligand-gated ion channel, 4
Synonyms ATP receptor, P2RX4, P2X purinoceptor 4, P2X4, P2X4R, Purinergic receptor
Calculated Molecular Weight 388 aa, 43 kDa
Observed Molecular Weight 45-60 kDa
GenBank Accession NumberBC033826
Gene Symbol P2RX4
Gene ID (NCBI) 5025
RRIDAB_2236528
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99571
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs). P2RX4 (P2X purinoceptor 4) is a receptor for ATP acting as a ligand gated ion channel. The calculated molecular weight of P2RX4 is 43 kDa, larger apparent molecular weights of 50-80 kDa have been reported, possibly due to post-translational glycosylation (PMID: 24040145, 15262999; 29382907; 12566439). The antibody can detect the band around 45 kDa.

实验方案

Product Specific Protocols
WB protocol for P2RX4 antibody 13534-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Autophagy

Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy

Authors - Kui Wang
mouseWB

Sci Adv

α-Ketoglutaric acid ameliorates hyperglycemia in diabetes by inhibiting hepatic gluconeogenesis via serpina1e signaling.

Authors - Yexian Yuan
  • KD Validated
humanIHC

Front Oncol

An Inflammatory Response-Related Gene Signature Can Impact the Immune Status and Predict the Prognosis of Hepatocellular Carcinoma.

Authors - Zhuo Lin
mouseWB

Exp Neurol

Neuroprotective and neuro-rehabilitative effects of acute purinergic receptor P2X4 (P2X4R) blockade after ischemic stroke.

Authors - Pranay Srivastava
humanIF

CNS Neurosci Ther

Activation of P2X4 receptor exacerbates acute brain injury after intracerebral hemorrhage.

Authors - Si-Ting Wu
mouseIHC

J Headache Pain

Microglia P2X4R-BDNF signalling contributes to central sensitization in a recurrent nitroglycerin-induced chronic migraine model.

Authors - Ting Long