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PAK2 Polyclonal antibody

PAK2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 19979-1-AP

Print datasheet

Synonyms

p21-activated kinase 2, p21 activated kinase 2, Gamma-PAK, Gamma PAK, EC:2.7.11.1

验证数据展示

  • IF Staining of HeLa using 19979-1-AP

    IF Staining of HeLa using 19979-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PAK2 antibody (19979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 19979-1-AP

    WB analysis using 19979-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19979-1-AP (PAK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 19979-1-AP

    WB analysis using 19979-1-AP

    Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 19979-1-AP (PAK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 19979-1-AP

    IP experiment of K-562 using 19979-1-AP

    IP result of anti-PAK2 (IP:19979-1-AP, 4ug; Detection:19979-1-AP 1:2000) with K-562 cells lysate 2400 ug.

经过测试的应用

Positive WB detected inHEK-293 cells, human skeletal muscle tissue, Jurkat cells, HeLa cells, PC-3 cells, Raji cells
Positive IP detected inK-562 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19979-1-AP targets PAK2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name p21 protein (Cdc42/Rac)-activated kinase 2
Synonyms p21-activated kinase 2, p21 activated kinase 2, Gamma-PAK, Gamma PAK, EC:2.7.11.1
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberNM_002577
Gene Symbol PAK2
Gene ID (NCBI) 5062
RRIDAB_10644144
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13177
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAK2, also named as PAK65, PAKgamma, p58, PAK-2p27, PAK-2p24 and C-t-PAK2, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. PAK2 has several isoforms with the MW of 54-62 kDa and 41 kDa. The 62 kDa PAK2 is cleaved into a 34 kDa C terminal fragment and a 28 kDa N terminal fragment with a time course that parallels apoptotic death in Jurkat cells. (PMID:10200518). Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form. The antibody has no cross reaction to PAK1 and PAK3.

实验方案

Product Specific Protocols
WB protocol for PAK2 antibody 19979-1-APDownload protocol
IF protocol for PAK2 antibody 19979-1-APDownload protocol
IP protocol for PAK2 antibody 19979-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols