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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated PARP1 Monoclonal antibody

PARP1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1D7D4

Cat no : CL647-66520

Print datasheet

Synonyms

ARTD1, ADPRT1, ADPRT 1, ADPRT, ADP-ribosyltransferase diphtheria toxin-like 1

验证数据展示

  • IF Staining of Neuro-2a using CL647-66520

    IF Staining of Neuro-2a using CL647-66520

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using CoraLite® Plus 647 PARP1 antibody (CL647-66520, Clone: 1D7D4 ) at dilution of 1:100.

  • FC experiment of HeLa using CL647-66520

    FC experiment of HeLa using CL647-66520

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human PARP1 (CL647-66520, Clone:1D7D4) (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inNeuro-2a cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PARP1 Ready-To-Use IHC Kit. PARP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66520-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-66520 targets PARP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen PARP1 fusion protein Ag19173 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name poly (ADP-ribose) polymerase 1
Synonyms ARTD1, ADPRT1, ADPRT 1, ADPRT, ADP-ribosyltransferase diphtheria toxin-like 1
Calculated Molecular Weight 1014 aa, 113 kDa
Observed Molecular Weight 113-116 kDa, 85-89 kDa
GenBank Accession NumberBC037545
Gene Symbol PARP1
Gene ID (NCBI) 142
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP09874
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the N-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 PARP1 antibody CL647-66520Download protocol
FC protocol for CL Plus 647 PARP1 antibody CL647-66520Download protocol
Standard Protocols
Click here to view our Standard Protocols