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PAX2 Polyclonal antibody

PAX2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 29307-1-AP

Print datasheet

Synonyms

paired box 2, Paired box protein Pax 2, PAX2

验证数据展示

  • WB analysis using 29307-1-AP

    WB analysis using 29307-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29307-1-AP (PAX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 29307-1-AP

    IHC staining of mouse kidney using 29307-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29307-1-AP (PAX2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAX2 antibody included
  • IHC staining of mouse kidney using 29307-1-AP

    IHC staining of mouse kidney using 29307-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29307-1-AP (PAX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAX2 antibody included

经过测试的应用

Positive WB detected inmouse cerebellum tissue, rat cerebellum tissue
Positive IHC detected inmouse kidney tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PAX2 Ready-To-Use IHC Kit. PAX2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29307-1-AP targets PAX2 in WB, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsFC, IHC
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Immunogen PAX2 fusion protein Ag30717 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name paired box 2
Synonyms paired box 2, Paired box protein Pax 2, PAX2
Calculated Molecular Weight 431 aa, 46 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC148710
Gene Symbol PAX2
Gene ID (NCBI) 5076
RRIDAB_2918278
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02962
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAX2 is a transcription factor, primarily expressed during embryonic development. It has a critical role in the development of the urogenital tract, the eyes, and the CNS. It plays a key role during renal development and to act as an oncogene favoring renal tumor growth. PAX2 was suggested as a sensitive and highly specific marker for renal cell carcinoma (RCC).

实验方案

Product Specific Protocols
WB protocol for PAX2 antibody 29307-1-APDownload protocol
IHC protocol for PAX2 antibody 29307-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Sci Bull (Beijing)

Reprogramming of Ovarian Granulosa Cells by YAP1 Leads to Development of High-Grade Cancer with Mesenchymal Lineage and Serous Features.

Authors - Xiangmin Lv
View Article
humanFC

Cells

Human iPSC-Derived Renal Cells Change Their Immunogenic Properties during Maturation: Implications for Regenerative Therapies.

Authors - Bella Rossbach
View Article