验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, A431 cells, HeLa cells, HEK-293 cells, PC-3 cells, human saliva, mouse heart tissue, mouse lung tissue, rat brain tissue, U-937 cells, THP-1 cells, RAW 264.7 cells |
| Positive IHC detected in | human pancreas cancer tissue, human kidney tissue, human lung cancer tissue, human ovary tumor tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18410-1-AP targets Progranulin/PGRN in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Progranulin/PGRN fusion protein Ag13178 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | granulin |
| 别名 | granulin, Granulin-1, Granulin precursor, Glycoprotein of 88 Kda, Glycoprotein 88 |
| 计算分子量 | 64 kDa |
| 观测分子量 | 60-70 kDa |
| GenBank蛋白编号 | BC000324 |
| 基因名称 | Granulin |
| Gene ID (NCBI) | 2896 |
| RRID | AB_10598161 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P28799 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa form. PGRN is a unique growth factor that plays an important role in cutaneous wound healing. It has an anti-inflammatory effect and promotes cell proliferation. When PCDGF is degraded to several 6-25 kDa fragments, called granulins (GRNs) by neutrophil proteases, a pro-inflammatory reaction occurs. PGRN is widely expressed, particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and its expression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding for pro-granulin (GRN) cause frontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest in progranulin and its functions in the central nervous system.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Progranulin/PGRN antibody 18410-1-AP | Download protocol |
| IHC protocol for Progranulin/PGRN antibody 18410-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Transl Psychiatry Progranulin improves neural development via the PI3K/Akt/GSK-3β pathway in the cerebellum of a VPA-induced rat model of ASD. | ||
J Proteome Res Mining the gastric cancer secretome: identification of GRN as a potential diagnostic marker for early gastric cancer. | ||
J Mol Med (Berl) Progranulin alleviates podocyte injury via regulating CAMKK/AMPK-mediated autophagy under diabetic conditions. | ||
Front Oncol GRN is a prognostic biomarker and correlated with immune infiltration in glioma: A study based on TCGA data | ||
Neuropharmacology Abnormal spatiotemporal expression pattern of progranulin and neurodevelopment impairment in VPA-induced ASD rat model. | ||
Int J Mol Sci Involvement of Progranulin and Granulin Expression in Inflammatory Responses after Cerebral Ischemia. |
