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PCNA Recombinant antibody, PBS Only

PCNA Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240306B9

Cat no : 81302-6-PBS

Print datasheet

Synonyms

proliferating cell nuclear antigen, Cyclin, 240306B9

验证数据展示

  • IHC staining of human stomach cancer using 81302-6-RR (same clone as 81302-6-PBS)

    IHC staining of human stomach cancer using 81302-6-RR (same clone as 81302-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81302-6-RR (PCNA antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81302-6-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PCNA antibody included
  • WB analysis using 81302-6-RR (same clone as 81302-6-PBS)

    WB analysis using 81302-6-RR (same clone as 81302-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81302-6-RR (PCNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81302-6-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 81302-6-RR (same clone as 81302-6-PBS)

    IHC staining of human stomach cancer using 81302-6-RR (same clone as 81302-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81302-6-RR (PCNA antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81302-6-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PCNA antibody included
  • IF Staining of mouse testis using 81302-6-RR (same clone as 81302-6-PBS)

    IF Staining of mouse testis using 81302-6-RR (same clone as 81302-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using PCNA antibody (81302-6-RR, Clone: 240306B9 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81302-6-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 81302-6-RR (same clone as 81302-6-PBS)

    FC experiment of HeLa using 81302-6-RR (same clone as 81302-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PCNA Recombinant antibody (81302-6-RR, Clone:240306B9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 81302-6-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PCNA Ready-To-Use IHC Kit. PCNA primary antibody included.

产品信息

The immunogen of 81302-6-PBS is PCNA Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF-P, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PCNA fusion protein Ag0277 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name proliferating cell nuclear antigen
Synonyms proliferating cell nuclear antigen, Cyclin, 240306B9
Calculated Molecular Weight 29 kDa/31 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC000491
Gene Symbol PCNA
Gene ID (NCBI) 5111
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP12004
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).