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验证数据展示

  • IHC staining of human stomach cancer using 81302-6-RR (same clone as 81302-6-PBS)

    IHC staining of human stomach cancer using 81302-6-RR (same clone as 81302-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81302-6-RR (PCNA antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81302-6-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PCNA antibody included
  • WB analysis using 81302-6-RR (same clone as 81302-6-PBS)

    WB analysis using 81302-6-RR (same clone as 81302-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81302-6-RR (PCNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81302-6-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 81302-6-RR (same clone as 81302-6-PBS)

    IHC staining of human stomach cancer using 81302-6-RR (same clone as 81302-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81302-6-RR (PCNA antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81302-6-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PCNA antibody included
  • IF Staining of mouse testis using 81302-6-RR (same clone as 81302-6-PBS)

    IF Staining of mouse testis using 81302-6-RR (same clone as 81302-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using PCNA antibody (81302-6-RR, Clone: 240306B9 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81302-6-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 81302-6-RR (same clone as 81302-6-PBS)

    FC experiment of HeLa using 81302-6-RR (same clone as 81302-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PCNA Recombinant antibody (81302-6-RR, Clone:240306B9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 81302-6-PBS in a different storage buffer formulation.

PCNA Recombinant antibody, PBS Only

PCNA Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Cat No. 81302-6-PBS

产品说明书

CloneNo. 240306B9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, FC (Intra), ELISA

别名

proliferating cell nuclear antigen, Cyclin, 240306B9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PCNA Ready-To-Use IHC Kit. PCNA primary antibody included.

产品信息

81302-6-PBS targets PCNA in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PCNA fusion protein Ag0277 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 proliferating cell nuclear antigen
别名 proliferating cell nuclear antigen, Cyclin, 240306B9
计算分子量 29 kDa/31 kDa
观测分子量 36 kDa
GenBank蛋白编号BC000491
基因名称 PCNA
Gene ID (NCBI) 5111
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP12004
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).