验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, Jurkat cells, HeLa cells, HepG2 cells, K-562 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue, human liver cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy PDCD6 Ready-To-Use IHC Kit. PDCD6 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12303-1-AP targets PDCD6/ALG2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | PDCD6/ALG2 fusion protein Ag2949 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | programmed cell death 6 |
| 别名 | ALG2, PDCD6, PEF1B, Apoptosis-linked gene 2 protein homolog, ALG-2 |
| 计算分子量 | 191 aa, 22 kDa |
| 观测分子量 | 15-22 kDa |
| GenBank蛋白编号 | BC012384 |
| 基因名称 | PDCD6 |
| Gene ID (NCBI) | 10016 |
| RRID | AB_2162459 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75340 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDCD6/ALG2 antibody 12303-1-AP | Download protocol |
| IHC protocol for PDCD6/ALG2 antibody 12303-1-AP | Download protocol |
| IP protocol for PDCD6/ALG2 antibody 12303-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Regulation of the CUL3 Ubiquitin Ligase by a Calcium-Dependent Co-adaptor.
| ||
Cell Rep Calcium-dependent ESCRT recruitment and lysosome exocytosis maintain epithelial integrity during Candida albicans invasion.
| ||
J Cell Biol Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters |
