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验证数据展示

  • WB analysis using 12303-1-AP

    WB analysis using 12303-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12303-1-AP (PDCD6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 12303-1-AP

    IHC staining of human lung cancer using 12303-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12303-1-AP (PDCD6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PDCD6 antibody included
  • IHC staining of human lung cancer using 12303-1-AP

    IHC staining of human lung cancer using 12303-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12303-1-AP (PDCD6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PDCD6 antibody included
  • IP experiment of HeLa using 12303-1-AP

    IP experiment of HeLa using 12303-1-AP

    IP result of anti-PDCD6 (IP:12303-1-AP, 3ug; Detection:12303-1-AP 1:500) with HeLa cells lysate 3200ug.

  • IHC staining of human stomach using 12303-1-AP

    IHC staining of human stomach using 12303-1-AP

    Immunohistochemical analysis of paraffin-embedded normal stomach slide using 12303-1-AP (PDCD6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PDCD6 antibody included
  • Featured Product
  • KD/KO验证

PDCD6/ALG2 Polyclonal antibody

PDCD6/ALG2 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 12303-1-AP

产品说明书

引用文献(22)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA and More (1)

别名

ALG2, PDCD6, PEF1B, Apoptosis-linked gene 2 protein homolog, ALG-2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, HeLa cells, HepG2 cells, K-562 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PDCD6 Ready-To-Use IHC Kit. PDCD6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12303-1-AP targets PDCD6/ALG2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 PDCD6/ALG2 fusion protein Ag2949 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 programmed cell death 6
别名 ALG2, PDCD6, PEF1B, Apoptosis-linked gene 2 protein homolog, ALG-2
计算分子量 191 aa, 22 kDa
观测分子量 15-22 kDa
GenBank蛋白编号BC012384
基因名称 PDCD6
Gene ID (NCBI) 10016
RRIDAB_2162459
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75340
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PDCD6/ALG2 antibody 12303-1-APDownload protocol
IHC protocol for PDCD6/ALG2 antibody 12303-1-APDownload protocol
IP protocol for PDCD6/ALG2 antibody 12303-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Structural basis for dimerization quality control.

Authors - Elijah L Mena
View Article
humanIF

Science

Triggered recruitment of ESCRT machinery promotes endolysosomal repair.

Authors - Michael L Skowyra
View Article
humanWB,IF

Cell

Regulation of the CUL3 Ubiquitin Ligase by a Calcium-Dependent Co-adaptor.

Authors - Colleen A McGourty
  • KD Validated
View Article
humanWB

Mol Cell

Co-adaptor driven assembly of a CUL3 E3 ligase complex.

Authors - David Akopian
View Article
humanWB,IF

Cell Rep

Calcium-dependent ESCRT recruitment and lysosome exocytosis maintain epithelial integrity during Candida albicans invasion.

Authors - Johannes Westman
  • KD Validated
View Article
humanIF

J Cell Biol

Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters

Authors - Femmy C Stempels
View Article