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ERp72 Polyclonal antibody

ERp72 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 14712-1-AP

Print datasheet

Synonyms

Protein disulfide-isomerase A4, PDIA4, ERp-72, ERp70, ERp 72

验证数据展示

  • WB analysis using 14712-1-AP

    WB analysis using 14712-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14712-1-AP (ERp72 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 14712-1-AP

    IHC staining of human liver cancer using 14712-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14712-1-AP (ERp72 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PDIA4/ERP72 antibody included
  • IHC staining of human liver cancer using 14712-1-AP

    IHC staining of human liver cancer using 14712-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14712-1-AP (ERp72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PDIA4/ERP72 antibody included
  • IF Staining of A431 using 14712-1-AP

    IF Staining of A431 using 14712-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using ERp72 antibody (14712-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IP experiment of HeLa using 14712-1-AP

    IP experiment of HeLa using 14712-1-AP

    IP result of anti-ERp72 (IP:14712-1-AP, 4ug; Detection:14712-1-AP 1:500) with HeLa cells lysate 2800ug.

  • FC experiment of HepG2 using 14712-1-AP

    FC experiment of HepG2 using 14712-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human ERp72 (14712-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells, HepG2 cells, L02 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, PC-3 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PDIA4/ERP72 Ready-To-Use IHC Kit. PDIA4/ERP72 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14712-1-AP targets ERp72 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig, monkey
Immunogen ERp72 fusion protein Ag6431 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein disulfide isomerase family A, member 4
Synonyms Protein disulfide-isomerase A4, PDIA4, ERp-72, ERp70, ERp 72
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC000425
Gene Symbol ERp72
Gene ID (NCBI) 9601
RRIDAB_2160973
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13667
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDIA4(Protein disulfide-isomerase A4) is also named as ERP70, ERP72 and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full length protein has a signal peptide with 20 amino acid.

实验方案

Product Specific Protocols
WB protocol for ERp72 antibody 14712-1-APDownload protocol
IHC protocol for ERp72 antibody 14712-1-APDownload protocol
IF protocol for ERp72 antibody 14712-1-APDownload protocol
IP protocol for ERp72 antibody 14712-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell

MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.

Authors - Xiaorong Zhang
View Article
humanWB

J Thromb Haemost

SINE-VNTR-Alu retrotransposon insertion as a novel mutational event underlying Glanzmann thrombasthenia

Authors - Jiasheng Zhang
View Article
humanWB

Mol Syst Biol

Time-resolved interactome profiling deconvolutes secretory protein quality control dynamics

Authors - Madison T Wright
View Article

Elife

Small molecule proteostasis regulators that reprogram the ER to reduce extracellular protein aggregation.

Authors - Lars Plate
View Article
humanWB

Cell Chem Biol

Quantitative Interactome Proteomics Reveals a Molecular Basis for ATF6-Dependent Regulation of a Destabilized Amyloidogenic Protein.

Authors - Lars Plate
View Article
mouseWB

Elife

PDIA1/P4HB is required for efficient proinsulin maturation and ß cell health in response to diet induced obesity.

Authors - Insook Jang
View Article