验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, THP-1 cells, human placenta tissue |
Positive IHC detected in | human liver cancer tissue, human tonsillitis tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:2000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:400-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66065-1-Ig targets CD31 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IF, IHC, ELISA Application Description |
文献引用应用 | WB, IF, IHC |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | CD31 fusion protein Ag1787 种属同源性预测 |
宿主/亚型 | Mouse / IgG2b |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | platelet/endothelial cell adhesion molecule |
别名 | |
计算分子量 | 83 kDa |
观测分子量 | 120-130 kDa |
GenBank蛋白编号 | BC022512 |
基因名称 | CD31 |
Gene ID (NCBI) | 5175 |
ENSEMBL Gene ID | ENSG00000261371 |
RRID | AB_11042590 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P16284 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).
实验方案
Product Specific Protocols | |
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WB protocol for CD31 antibody 66065-1-Ig | Download protocol |
IHC protocol for CD31 antibody 66065-1-Ig | Download protocol |
IF protocol for CD31 antibody 66065-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
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