CD31 Monoclonal antibody

CD31 Monoclonal Antibody for WB, IF, IHC, ELISA
Cat No. 66065-1-Ig

产品说明书

CloneNo. 2A1E2

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IF, IHC, ELISA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, THP-1 cells, human placenta tissue
Positive IHC detected inhuman liver cancer tissue, human tonsillitis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD31 Ready-To-Use IHC Kit. CD31 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66065-1-Ig targets CD31 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF, IHC, ELISA Application Description
文献引用应用WB, IF, IHC
经测试反应性 human
文献引用反应性human
免疫原 CD31 fusion protein Ag1787 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 platelet/endothelial cell adhesion molecule
别名
计算分子量 83 kDa
观测分子量 120-130 kDa
GenBank蛋白编号BC022512
基因名称 CD31
Gene ID (NCBI) 5175
ENSEMBL Gene IDENSG00000261371
RRIDAB_11042590
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP16284
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

实验方案

Product Specific Protocols
WB protocol for CD31 antibody 66065-1-IgDownload protocol
IHC protocol for CD31 antibody 66065-1-IgDownload protocol
IF protocol for CD31 antibody 66065-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nature

Construction of a human cell landscape at single-cell level.

Authors - Xiaoping Han

Biomaterials

An iRGD-conjugated prodrug micelle with blood-brain-barrier penetrability for anti-glioma therapy.

Authors - Lu Lu
humanIF

Small

Airflow-Assisted 3D Bioprinting of Human Heterogeneous Microspheroidal Organoids with Microfluidic Nozzle.

Authors - Haiming Zhao
humanIF

Theranostics

Single-cell landscape reveals active cell subtypes and their interaction in the tumor microenvironment of gastric cancer.

Authors - Yumin Li
IF

Theranostics

Encapsulation of LXR ligand by D-Nap-GFFY hydrogel enhances anti-tumorigenic actions of LXR and removes LXR-induced lipogenesis.

Authors - Ke Feng
IHC

Cancer Lett

CD44(+) tumor cells promote early angiogenesis in head and neck squamous cell carcinoma.

Authors - Nils Ludwig
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