验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IHC | See 1 publications below |
产品信息
20662-1-AP targets PI3 Kinase p110 Gamma in WB, IHC, IP, ELISA applications and shows reactivity with mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
fusion protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphoinositide-3-kinase, catalytic, gamma polypeptide |
| 别名 | PIK3CG, EC:2.7.1.137, EC:2.7.11.1, p110gamma, p120 PI3K |
| 计算分子量 | 126 kDa |
| 观测分子量 | 100-130 kDa |
| GenBank蛋白编号 | NM_002649 |
| 基因名称 | PIK3CG |
| Gene ID (NCBI) | 5294 |
| RRID | AB_2878720 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P48736 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PI3 Kinase p110 Gamma antibody 20662-1-AP | Download protocol |
| IHC protocol for PI3 Kinase p110 Gamma antibody 20662-1-AP | Download protocol |
| IP protocol for PI3 Kinase p110 Gamma antibody 20662-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Physiol Endocrinol Metab Frequent and mild scrotal heat stress in mice epigenetically alters glucose metabolism in the male offspring. | ||
Am J Pathol Suppression of Overactive Insulin-Like Growth Factor 1 Attenuates Trauma-Induced Heterotopic Ossification in Mice | ||
Int J Biol Macromol Water-insoluble dietary fiber from walnut relieves constipation through Limosilactobacillus reuteri-mediated serotonergic synapse and neuroactive ligand-receptor pathways | ||
Biofactors Knockdown of integrin β1 inhibits proliferation and promotes apoptosis in bladder cancer cells | ||
Nat Commun Microbiota-indole-3-propionic acid-heart axis mediates the protection of leflunomide against αPD1-induced cardiotoxicity in mice |