验证数据展示
经过测试的应用
Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 3 publications below |
IHC | See 1 publications below |
产品信息
20662-1-AP targets PI3 Kinase p110 Gamma in WB, IHC, IP, ELISA applications and shows reactivity with Rat, mouse samples.
经测试应用 | WB, IP, IHC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | Rat, mouse |
文献引用反应性 | human, mouse |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | phosphoinositide-3-kinase, catalytic, gamma polypeptide |
别名 | p120 PI3K, PI3 Kinase p110 Gamma, PI3 kinase subunit gamma, PI3CG, PI3K, PI3K gamma, PI3K p110(gamma), PI3Kgamma, PIK3, PIK3CG, PtdIns 3 kinase subunit gamma |
计算分子量 | 126 kDa |
观测分子量 | 100-130 kDa |
GenBank蛋白编号 | NM_002649 |
基因名称 | PIK3CG |
Gene ID (NCBI) | 5294 |
RRID | AB_2878720 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P48736 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for PI3 Kinase p110 Gamma antibody 20662-1-AP | Download protocol |
IHC protocol for PI3 Kinase p110 Gamma antibody 20662-1-AP | Download protocol |
IP protocol for PI3 Kinase p110 Gamma antibody 20662-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Am J Physiol Endocrinol Metab Frequent and mild scrotal heat stress in mice epigenetically alters glucose metabolism in the male offspring. | ||
Am J Pathol Suppression of Overactive Insulin-Like Growth Factor 1 Attenuates Trauma-Induced Heterotopic Ossification in Mice | ||
Int J Biol Macromol Water-insoluble dietary fiber from walnut relieves constipation through Limosilactobacillus reuteri-mediated serotonergic synapse and neuroactive ligand-receptor pathways | ||
Biofactors Knockdown of integrin β1 inhibits proliferation and promotes apoptosis in bladder cancer cells |