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PI3 Kinase p110 Gamma Polyclonal antibody

PI3 Kinase p110 Gamma Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Rat, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20662-1-AP

Print datasheet

Synonyms

p120 PI3K, PI3 Kinase p110 Gamma, PI3 kinase subunit gamma, PI3CG, PI3K, PI3K gamma, PI3K p110(gamma), PI3Kgamma, PIK3, PIK3CG, PtdIns 3 kinase subunit gamma

验证数据展示

  • WB analysis using 20662-1-AP

    WB analysis using 20662-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20662-1-AP (PI3 Kinase p110 Gamma antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 20662-1-AP

    IP experiment of mouse liver using 20662-1-AP

    IP result of anti-PI3 Kinase p110 Gamma (IP:20662-1-AP, 5ug; Detection:20662-1-AP 1:300) with mouse liver tissue lysate 4000ug.

  • IHC staining of mouse skeletal muscle using 20662-1-AP

    IHC staining of mouse skeletal muscle using 20662-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20662-1-AP (PI3 Kinase p110 Gamma antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with .

  • IHC staining of mouse skeletal muscle using 20662-1-AP

    IHC staining of mouse skeletal muscle using 20662-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20662-1-AP (PI3 Kinase p110 Gamma antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with .

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

20662-1-AP targets PI3 Kinase p110 Gamma in WB, IP, IHC, ELISA applications and shows reactivity with Rat, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Rat, mouse
Cited Reactivitymouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphoinositide-3-kinase, catalytic, gamma polypeptide
Synonyms p120 PI3K, PI3 Kinase p110 Gamma, PI3 kinase subunit gamma, PI3CG, PI3K, PI3K gamma, PI3K p110(gamma), PI3Kgamma, PIK3, PIK3CG, PtdIns 3 kinase subunit gamma
Calculated Molecular Weight 126 kDa
Observed Molecular Weight 100-130 kDa
GenBank Accession NumberNM_002649
Gene Symbol PIK3CG
Gene ID (NCBI) 5294
RRIDAB_2878720
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48736
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PI3 Kinase p110 Gamma antibody 20662-1-APDownload protocol
IHC protocol for PI3 Kinase p110 Gamma antibody 20662-1-APDownload protocol
IP protocol for PI3 Kinase p110 Gamma antibody 20662-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Am J Physiol Endocrinol Metab

Frequent and mild scrotal heat stress in mice epigenetically alters glucose metabolism in the male offspring.

Authors - Xiaoyan Wan
View Article
mouseWB

Am J Pathol

Suppression of Overactive Insulin-Like Growth Factor 1 Attenuates Trauma-Induced Heterotopic Ossification in Mice

Authors - Dong Mao
View Article