验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
20662-1-AP targets PI3 Kinase p110 Gamma in WB, IHC, IP, ELISA applications and shows reactivity with Rat, mouse samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | Rat, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | phosphoinositide-3-kinase, catalytic, gamma polypeptide |
| Synonyms | p120 PI3K, PI3 Kinase p110 Gamma, PI3 kinase subunit gamma, PI3CG, PI3K, PI3K gamma, PI3K p110(gamma), PI3Kgamma, PIK3, PIK3CG, PtdIns 3 kinase subunit gamma |
| Calculated Molecular Weight | 126 kDa |
| Observed Molecular Weight | 100-130 kDa |
| GenBank Accession Number | NM_002649 |
| Gene Symbol | PIK3CG |
| Gene ID (NCBI) | 5294 |
| RRID | AB_2878720 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P48736 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PI3 Kinase p110 Gamma antibody 20662-1-AP | Download protocol |
| IHC protocol for PI3 Kinase p110 Gamma antibody 20662-1-AP | Download protocol |
| IP protocol for PI3 Kinase p110 Gamma antibody 20662-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Physiol Endocrinol Metab Frequent and mild scrotal heat stress in mice epigenetically alters glucose metabolism in the male offspring. | ||
Am J Pathol Suppression of Overactive Insulin-Like Growth Factor 1 Attenuates Trauma-Induced Heterotopic Ossification in Mice | ||
Int J Biol Macromol Water-insoluble dietary fiber from walnut relieves constipation through Limosilactobacillus reuteri-mediated serotonergic synapse and neuroactive ligand-receptor pathways | ||
Biofactors Knockdown of integrin β1 inhibits proliferation and promotes apoptosis in bladder cancer cells |