• Featured Product
  • KD/KO验证

VPS34 (C terminal) Polyclonal antibody

VPS34 (C terminal) Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12452-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (3)

PIK3C3, Vps34, EC:2.7.1.137, hVps34, PI 3 Kinase Class 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inPC-3 cells, mouse brain tissue, HeLa cells, human testis tissue, human brain tissue, mouse lung tissue, mouse testis tissue, Jurkat cells, rat brain tissue
Positive IHC detected inhuman prostate cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy PIK3C3/VPS34 Ready-To-Use IHC Kit. PIK3C3/VPS34 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12452-1-AP targets VPS34 (C terminal) in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 VPS34 (C terminal) fusion protein Ag3110 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphoinositide-3-kinase, class 3
别名 PIK3C3, Vps34, EC:2.7.1.137, hVps34, PI 3 Kinase Class 3
计算分子量 887 aa, 100 kDa
观测分子量 100 kDa
GenBank蛋白编号BC033004
基因名称 VPS34
Gene ID (NCBI) 5289
RRIDAB_2299709
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NEB9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIK3C3(Phosphatidylinositol 3-kinase catalytic subunit type 3) is also named as VPS34 and belongs to the PI3/PI4-kinase family.It is a catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes.It can form a dimer(PMID:20339072).

实验方案

Product Specific Protocols
WB protocol for VPS34 (C terminal) antibody 12452-1-APDownload protocol
IHC protocol for VPS34 (C terminal) antibody 12452-1-APDownload protocol
IF protocol for VPS34 (C terminal) antibody 12452-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cancer

Overcoming multi-drug resistance in SCLC: a synergistic approach with venetoclax and hydroxychloroquine targeting the lncRNA LYPLAL1-DT/BCL2/BECN1 pathway

Authors - Shuxin Li
mouseWB,IF

J Thromb Haemost

Vps34 derived phosphatidylinositol 3-monophosphate modulates megakaryocyte maturation and proplatelet production through late endosomes/lysosomes.

Authors - Ivana Bertović
humanWB

Mol Cancer

PMID: 32727463

Authors - Cefan Zhou
humanWB

Nat Commun

Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.

Authors - Wenjun Wang
humanWB

Nat Commun

Structural basis for VPS34 kinase activation by Rab1 and Rab5 on membranes.

Authors - Shirley Tremel
IP,CoIP

Nat Commun

IL-6 regulates autophagy and chemotherapy resistance by promoting BECN1 phosphorylation.

Authors - Fuqing Hu
Loading...