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- KD/KO Validated
PINK1 Polyclonal antibody
PINK1 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (6)
Applications
WB, IHC, IF-P, ELISA and More (3)
Conjugate
Unconjugated
319
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, CCCP treated HeLa cells, PC-12 cells |
Positive IHC detected in | mouse brain tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue, rat brain tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
23274-1-AP targets PINK1 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF-P, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, rabbit, monkey, zebrafish, goat, duck |
Immunogen | PINK1 fusion protein Ag19825 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | PTEN induced putative kinase 1 |
Synonyms | Serine/threonine-protein kinase PINK1, mitochondrial, PTEN-induced putative kinase protein 1, PTEN induced putative kinase 1, PARK6, EC:2.7.11.1 |
Calculated Molecular Weight | 581 aa, 63 kDa |
Observed Molecular Weight | 65 kDa, 45 kDa |
GenBank Accession Number | BC028215 |
Gene Symbol | PINK1 |
Gene ID (NCBI) | 65018 |
RRID | AB_2879244 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen Affinity purified |
UNIPROT ID | Q9BXM7 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PINK1 antibody 23274-1-AP | Download protocol |
IHC protocol for PINK1 antibody 23274-1-AP | Download protocol |
IF protocol for PINK1 antibody 23274-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Cell Biol Ammonia-induced lysosomal and mitochondrial damage causes cell death of effector CD8+ T cells | ||
J Hazard Mater Low-dose deoxynivalenol exposure inhibits hepatic mitophagy and hesperidin reverses this phenomenon by activating SIRT1 | ||
Nat Commun Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength. | ||
Redox Biol Atox1 protects hippocampal neurons after traumatic brain injury via DJ-1 mediated anti-oxidative stress and mitophagy |