验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, CCCP treated HeLa cells, PC-12 cells |
| Positive IHC detected in | mouse brain tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue, rat brain tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23274-1-AP targets PINK1 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF-P, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, rabbit, monkey, zebrafish, goat, duck |
| Immunogen | PINK1 fusion protein Ag19825 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | PTEN induced putative kinase 1 |
| Synonyms | Serine/threonine-protein kinase PINK1, mitochondrial, PTEN-induced putative kinase protein 1, PTEN induced putative kinase 1, PARK6, EC:2.7.11.1 |
| Calculated Molecular Weight | 581 aa, 63 kDa |
| Observed Molecular Weight | 65 kDa, 45 kDa |
| GenBank Accession Number | BC028215 |
| Gene Symbol | PINK1 |
| Gene ID (NCBI) | 65018 |
| RRID | AB_2879244 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q9BXM7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PINK1 is a mitochondrial serine/threonine-protein kinase that protects cells from stress-induced mitochondrial dysfunction. The precursor of PINK1 (65 kDa) is synthesized in the cytosol and is imported into the outer membrane of mitochondria. PINK1 is further transferred into the inner membrane. The full-length PINK1 can be proteolytically processed into 52-55 kDa and 45-46 kDa forms (PMID: 18221368; 25108683; 18031932). The half-life of the mature form of PINK1 is very short and it was proposed that the proteasome is involved in its degradation (PMID: 23472196). The gene of PINK1 maps to chromosome 1p36.12. Two alternatively spliced variants exist, the shorter isoform (30 kDa) produced by alternative splicing. Mutations in the PINK1 gene cause autosomal recessive early-onset Parkinson's disease.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PINK1 antibody 23274-1-AP | Download protocol |
| IHC protocol for PINK1 antibody 23274-1-AP | Download protocol |
| IF protocol for PINK1 antibody 23274-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Ammonia-induced lysosomal and mitochondrial damage causes cell death of effector CD8+ T cells | ||
Adv Sci (Weinh) WAC Facilitates Mitophagy-mediated MSC Osteogenesis and New Bone Formation via Protecting PINK1 from Ubiquitination-Dependent Degradation | ||
J Hazard Mater Low-dose deoxynivalenol exposure inhibits hepatic mitophagy and hesperidin reverses this phenomenon by activating SIRT1 | ||
Nat Commun Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength. |
