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PINK1 Polyclonal antibody

PINK1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF-P, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 23274-1-AP

Print datasheet

Synonyms

Serine/threonine-protein kinase PINK1, mitochondrial, PTEN-induced putative kinase protein 1, PTEN induced putative kinase 1, PARK6, EC:2.7.11.1



经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, CCCP treated HeLa cells, PC-12 cells
Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue, rat brain tissue
Planning an IHC experiment? We recommend our IHCeasy PINK1 Ready-To-Use IHC Kit. PINK1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23274-1-AP targets PINK1 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, rabbit, monkey, zebrafish, goat, duck
Immunogen PINK1 fusion protein Ag19825 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PTEN induced putative kinase 1
Synonyms Serine/threonine-protein kinase PINK1, mitochondrial, PTEN-induced putative kinase protein 1, PTEN induced putative kinase 1, PARK6, EC:2.7.11.1
Calculated Molecular Weight 581 aa, 63 kDa
Observed Molecular Weight 65 kDa, 45 kDa
GenBank Accession NumberBC028215
Gene Symbol PINK1
Gene ID (NCBI) 65018
RRIDAB_2879244
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9BXM7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PINK1 is a mitochondrial serine/threonine-protein kinase that protects cells from stress-induced mitochondrial dysfunction. The precursor of PINK1 (65 kDa) is synthesized in the cytosol and is imported into the outer membrane of mitochondria. PINK1 is further transferred into the inner membrane. The full-length PINK1 can be proteolytically processed into 52-55 kDa and 45-46 kDa forms (PMID: 18221368; 25108683; 18031932). The half-life of the mature form of PINK1 is very short and it was proposed that the proteasome is involved in its degradation (PMID: 23472196). The gene of PINK1 maps to chromosome 1p36.12. Two alternatively spliced variants exist, the shorter isoform (30 kDa) produced by alternative splicing. Mutations in the PINK1 gene cause autosomal recessive early-onset Parkinson's disease.

实验方案

Product Specific Protocols
WB protocol for PINK1 antibody 23274-1-APDownload protocol
IHC protocol for PINK1 antibody 23274-1-APDownload protocol
IF protocol for PINK1 antibody 23274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Cell Biol

Ammonia-induced lysosomal and mitochondrial damage causes cell death of effector CD8+ T cells

Authors - Huafeng Zhang
humanWB

Nat Commun

Gonococcal OMV-delivered PorB induces epithelial cell mitophagy

Authors - Shuai Gao
humanWB

Mol Cell

The HRI branch of the integrated stress response selectively triggers mitophagy

Authors - Yogaditya Chakrabarty
human,mouseWB,IP

J Hazard Mater

Low-dose deoxynivalenol exposure inhibits hepatic mitophagy and hesperidin reverses this phenomenon by activating SIRT1

Authors - Hao Chen
mouseWB

Nat Commun

Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.

Authors - Zhisheng Xu
mouseWB

Redox Biol

Atox1 protects hippocampal neurons after traumatic brain injury via DJ-1 mediated anti-oxidative stress and mitophagy

Authors - Pengzhan Zhao