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验证数据展示

  • WB analysis using 15278-1-AP

    WB analysis using 15278-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15278-1-AP (POLE3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 15278-1-AP

    IHC staining of human liver cancer using 15278-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 15278-1-AP (POLE3 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human liver cancer using 15278-1-AP

    IHC staining of human liver cancer using 15278-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 15278-1-AP (POLE3 antibody) at dilution of 1:100 (under 10x lens).

  • IP experiment of HepG2 using 15278-1-AP

    IP experiment of HepG2 using 15278-1-AP

    IP result of anti-POLE3 (IP:15278-1-AP, 4ug; Detection:15278-1-AP 1:1000) with HepG2 cells lysate 1800ug.

  • IF Staining of HepG2 using 15278-1-AP

    IF Staining of HepG2 using 15278-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15278-1-AP (POLE3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

POLE3 Polyclonal antibody

POLE3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15278-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

CHRAC17, CHRAC 17, CHARAC17, AsTP, Arsenic-transactivated protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15278-1-AP targets POLE3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 POLE3 fusion protein Ag7396 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polymerase (DNA directed), epsilon 3 (p17 subunit)
别名 CHRAC17, CHRAC 17, CHARAC17, AsTP, Arsenic-transactivated protein
计算分子量 17 kDa
观测分子量 17 kDa
GenBank蛋白编号BC003166
基因名称 POLE3
Gene ID (NCBI) 54107
RRIDAB_2166751
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRF9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for POLE3 antibody 15278-1-APDownload protocol
IHC protocol for POLE3 antibody 15278-1-APDownload protocol
IF protocol for POLE3 antibody 15278-1-APDownload protocol
IP protocol for POLE3 antibody 15278-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Biochem Cell Biol

SNORA42 enhances prostate cancer cell viability, migration and EMT and is correlated with prostate cancer poor prognosis.

Authors - Chuanyou Yi
View Article
humanWB

Front Oncol

ASF1B Promotes Oncogenesis in Lung Adenocarcinoma and Other Cancer Types.

Authors - Wencheng Zhang
View Article