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  • Featured Product
  • KD/KO Validated

POM121 Polyclonal antibody

POM121 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15645-1-AP

Print datasheet

Synonyms

POM121A, NUP121, Nucleoporin Nup121, Nuclear envelope pore membrane protein POM 121A, Nuclear envelope pore membrane protein POM 121

验证数据展示

  • WB analysis using 15645-1-AP

    WB analysis using 15645-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15645-1-AP (POM121 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 15645-1-AP

    IF Staining of mouse brain using 15645-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 15645-1-AP (POM121 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon using 15645-1-AP

    IHC staining of human colon using 15645-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15645-1-AP (POM121 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15645-1-AP targets POM121 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen POM121 fusion protein Ag8177 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name POM121 membrane glycoprotein (rat)
Synonyms POM121A, NUP121, Nucleoporin Nup121, Nuclear envelope pore membrane protein POM 121A, Nuclear envelope pore membrane protein POM 121
Calculated Molecular Weight 984 aa, 99 kDa
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberBC008794
Gene Symbol POM121
Gene ID (NCBI) 9883
RRIDAB_2878160
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96HA1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POM121 is an integral membrane protein that localizes to the central spoke ring complex and participates in anchoring the nuclear pore complex to the nuclear envelope. Catalog#15645-1-AP do well in identifying the nuclear envelope in immunofluorescence experiments.

实验方案

Product Specific Protocols
WB protocol for POM121 antibody 15645-1-APDownload protocol
IHC protocol for POM121 antibody 15645-1-APDownload protocol
IF protocol for POM121 antibody 15645-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nature

Heritable transcriptional defects from aberrations of nuclear architecture

Authors - Stamatis Papathanasiou
View Article
humanIF

Nat Neurosci

Interactions between ALS-linked FUS and nucleoporins are associated with defects in the nucleocytoplasmic transport pathway.

Authors - Yen-Chen Lin
View Article
humanIF

Dev Cell

A tubule-sheet continuum model for the mechanism of nuclear envelope assembly

Authors - Gengjing Zhao
View Article
humanWB

Elife

Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.

Authors - Melissa Kane
View Article
humanIHC,WB

Cancer Biomark

POM121 promotes proliferation and metastasis in non-small-cell lung cancer through TGF-β/SMAD and PI3K/AKT pathways

Authors - Lirong Guan
  • KD Validated
View Article
humanIF

bioRxiv

Altered nuclear envelope homeostasis is a key pathogenic event in C9ORF72-linked ALS/FTD

Authors - Riccardo Sirtori
View Article