验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IHC detected in | mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
IF | See 1 publications below |
产品信息
10748-1-AP targets DARPP32 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | mouse |
免疫原 | DARPP32 fusion protein Ag1216 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | protein phosphatase 1, regulatory (inhibitor) subunit 1B |
别名 | DARPP32/PPP1R1B, PPP1R1B, Dopamine- and cAMP-regulated neuronal phosphoprotein, DARPP-32 |
计算分子量 | 23 kDa |
观测分子量 | 32 kDa |
GenBank蛋白编号 | BC001519 |
基因名称 | DARPP32 |
Gene ID (NCBI) | 84152 |
RRID | AB_2300051 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9UD71 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Dopamine- and cAMP-regulated neuronal phosphoprotein (DARPP32) , also known as protein phosphatase 1 regulatory subunit 1B (PPP1R1B), is a substrate of cAMP-dependent protein kinase (PKA) enriched in dopamine-innervated brain areas. Overexpression of DARPP32 has been shown to block gefitinib-induced apoptosis in gastric tumors by promoting EGFR and ERBB3 heterodimerization (PMID: 21741919). DARPP32 also drives gefitinib-refractory lung tumor growth through similar ERBB3-mediated resistance mechanisms(PMID: 34675407). The molecular weight of DARPP32 is about 32 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for DARPP32 antibody 10748-1-AP | Download protocol |
IHC protocol for DARPP32 antibody 10748-1-AP | Download protocol |
IF protocol for DARPP32 antibody 10748-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Acta Pharmacol Sin Ginsenoside Rg1 exerts neuroprotective effects in 3-nitropronpionic acid-induced mouse model of Huntington's disease via suppressing MAPKs and NF-κB pathways in the striatum. | ||
CNS Neurosci Ther A New VMAT-2 Inhibitor NBI-641449 in the Treatment of Huntington Disease. | ||
Free Radic Biol Med Disruption of Cdk5-GluN2B complex by a small interfering peptide attenuates social isolation-induced escalated intermale attack behavior and hippocampal oxidative stress in mice |