DARPP32 Polyclonal antibody

DARPP32 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 10748-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, ELISA and More (1)

DARPP32/PPP1R1B, PPP1R1B, Dopamine- and cAMP-regulated neuronal phosphoprotein, DARPP-32

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10748-1-AP targets DARPP32 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 DARPP32 fusion protein Ag1216 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 1, regulatory (inhibitor) subunit 1B
别名 DARPP32/PPP1R1B, PPP1R1B, Dopamine- and cAMP-regulated neuronal phosphoprotein, DARPP-32
计算分子量 23 kDa
观测分子量 32 kDa
GenBank蛋白编号BC001519
基因名称 DARPP32
Gene ID (NCBI) 84152
RRIDAB_2300051
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UD71
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dopamine- and cAMP-regulated neuronal phosphoprotein (DARPP32) , also known as protein phosphatase 1 regulatory subunit 1B (PPP1R1B), is a substrate of cAMP-dependent protein kinase (PKA) enriched in dopamine-innervated brain areas. Overexpression of DARPP32 has been shown to block gefitinib-induced apoptosis in gastric tumors by promoting EGFR and ERBB3 heterodimerization (PMID: 21741919). DARPP32 also drives gefitinib-refractory lung tumor growth through similar ERBB3-mediated resistance mechanisms(PMID: 34675407). The molecular weight of DARPP32 is about 32 kDa.

实验方案

Product Specific Protocols
WB protocol for DARPP32 antibody 10748-1-APDownload protocol
IHC protocol for DARPP32 antibody 10748-1-APDownload protocol
IF protocol for DARPP32 antibody 10748-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Acta Pharmacol Sin

Ginsenoside Rg1 exerts neuroprotective effects in 3-nitropronpionic acid-induced mouse model of Huntington's disease via suppressing MAPKs and NF-κB pathways in the striatum.

Authors - Xiong Yang
mouseIF

CNS Neurosci Ther

A New VMAT-2 Inhibitor NBI-641449 in the Treatment of Huntington Disease.

Authors - Sheng Chen
mouseWB

Free Radic Biol Med

Disruption of Cdk5-GluN2B complex by a small interfering peptide attenuates social isolation-induced escalated intermale attack behavior and hippocampal oxidative stress in mice

Authors - Heng Ai
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