验证数据展示
经过测试的应用
Positive ELISA detected in | peptide |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
23979-1-AP targets PR repeat in WB, IHC, IF, Dot Blot, ELISA applications and shows reactivity with human samples.
经测试应用 | ELISA Application Description |
文献引用应用 | WB, IHC, IF, Dot Blot |
经测试反应性 | human |
文献引用反应性 | human, mouse, rat, drosophila |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | PR repeat |
别名 | C9RAN, GGGCC, poly PR, poly-PR, PR repeat, PRPRPRPRP |
基因名称 | |
Gene ID (NCBI) | |
RRID | AB_2879388 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446).This antibody is used to detect the "PR repeat" sequence. This antibody detects the PRPRPRPRP peptide with dilution 1:4,500 in Elisa.
发表文章
Species | Application | Title |
---|---|---|
Nat Neurosci RPS25 is required for efficient RAN translation of C9orf72 and other neurodegenerative disease-associated nucleotide repeats. | ||
Neuron Antisense Proline-Arginine RAN Dipeptides Linked to C9ORF72-ALS/FTD Form Toxic Nuclear Aggregates that Initiate In Vitro and In Vivo Neuronal Death. | ||
Nat Commun Motor dysfunction and neurodegeneration in a C9orf72 mouse line expressing poly-PR. | ||
Acta Neuropathol Quantitative analysis and clinico-pathological correlations of different dipeptide repeat protein pathologies in C9ORF72 mutation carriers. | ||
EMBO J NDST3 deacetylates α-tubulin and suppresses V-ATPase assembly and lysosomal acidification. | ||
EMBO J The RNA exosome complex degrades expanded hexanucleotide repeat RNA in C9orf72 FTLD/ALS. |