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- KD/KO Validated
AMPK Alpha Polyclonal antibody
AMPK Alpha Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (6)
Applications
IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
184
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, Jurkat cells, NIH/3T3 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10929-2-AP targets AMPK Alpha in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IHC, IP, WB, ELISA |
Cited Applications | IF, IHC, IP, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, goat, carp, rabbit, fish, zebrafish, pig |
Immunogen | AMPK Alpha fusion protein Ag1367 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | protein kinase, AMP-activated, alpha 1 catalytic subunit |
Synonyms | AMPK, AMPK alpha, AMPK alpha 1, AMPK subunit alpha 1, AMPK1, AMPKa1, PRKAA1 |
Calculated Molecular Weight | 63 kDa |
Observed Molecular Weight | 64 kDa |
GenBank Accession Number | BC012622 |
Gene Symbol | PRKAA1 |
Gene ID (NCBI) | 5562 |
RRID | AB_2169568 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q13131 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for AMPK Alpha antibody 10929-2-AP | Download protocol |
IHC protocol for AMPK Alpha antibody 10929-2-AP | Download protocol |
IP protocol for AMPK Alpha antibody 10929-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Autophagy Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy | ||
Autophagy The AMPK-MFN2 axis regulates MAM dynamics and autophagy induced by energy stresses.
| ||
Autophagy A plant nonenveloped double-stranded RNA virus activates and co-opts BNIP3-mediated mitophagy to promote persistent infection in its insect vector. | ||
J Hazard Mater Mitigation of honokiol on fluoride-induced mitochondrial oxidative stress, mitochondrial dysfunction, and cognitive deficits through activating AMPK/PGC-1α/Sirt3. | ||
J Hazard Mater Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells. |